Abstract

Background
At a tertiary infertility center, we depicted major differences in endometrial microbiome composition, depending on stage of in vitro fertilization (IVF) treatment and pregnancy outcome. Yet, because of potential vaginal contamination of endometrial biopsies and technical limitations of metagenomics, we propose a novel approach in studying the vagino-uterine microbiome. Culturomics is a high-throughput culturing approach that uses multiple culture conditions and matrix-assisted laser desorption/ionization-time of flight mass spectrophotometry (MALDI-TOF MS). WASPLab® application guarantees perfect traceability and reduces the high workload of culturomics. As such, we investigate to what extent the endometrial microbiome corresponds to that of the vagina with culturomics and WASPLab®.


Methods
Paired samples of endometrial biopsy and vaginal swab were simultaneously obtained from four subfertile women undergoing diagnostic hysteroscopy. For pre-incubation of these samples, an aerobic and anaerobic blood culture bottle with sterile rumen fluid, sterile defibrinated sheep blood and a homemade supplement mix were used. After pre-incubation, seven different agar plates were inoculated and incubated aerobic (four plates) and anaerobic (three plates) on different days: 1, 5, 10 and 30. A WASPLab®-system was used to process the samples.


Results
A total of 1872 colonies were identified using the MALDI Biotyper® system enabling us to identify 55 different species. In the first, second and fourth patient, we found 13, 11 and 17 different species of which six (46.2%), four (36.4%) and seven (41.2%) were present in both vaginal and endometrial samples. These patients were colonized with Lactobacillus crispatus, Lactobacillus jensenii and/or Lactobacillus gasseri. In the third patient, we found 39 different species, of which two (5.1%) were present in both vaginal and endometrial samples. This patient’s vagina was colonized with Lactobacillus iners and 34 other species such as Candida albicans and Gardnerella vaginalis.


Conclusions
Our results suggest the existence of different endometrial and vaginal culturomics-microbiome profiles. In three of the patients we observed a eubiosis-related vaginal microbiome profile, while a dysbiotic vaginal microbiome was observed in the fourth woman. These preliminary findings seem associated with microbial diversity and presence of certain Lactobacillus species both at vaginal and endometrial level.
Original languageEnglish
Publication statusPublished - 2022
Event32nd European Congress of Clinical Microbiology & Infectious Diseases (ECCMID) - Lisbon, Portugal
Duration: 23 Apr 202226 Apr 2022
https://www.eccmid.org/

Conference

Conference32nd European Congress of Clinical Microbiology & Infectious Diseases (ECCMID)
CountryPortugal
CityLisbon
Period23/04/2226/04/22
Internet address

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