Comprehensive Biomedical physics: Bioluminescence Imaging

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Bioluminescence imaging Bioluminescence imaging is a research technique that uses light-producing enzymes from naturally occurring species or modified versions of these enzymes to follow biological processes inside cells or animals. The luciferase enzyme is often encoded in a reporter gene that is introduced into the cells or animals of interest. The amount of light that is detected by a light-sensitive
camera represents changes in the chosen biological process.
In vivo Literally ‘in the living.’ Here, in vivo is used to refer to experiment performed in living animals.
In vitro Literally ‘in glass.’ When experiments are performed in a laboratory vessel rather than a living organism. Here, all experiments performed outside a living animal are referred to as in vitro, although some authors still regard intact cell assays as in vivo since it still concerns living cells.
Original languageEnglish
Title of host publicationComprehensive Biomedical physics
Subtitle of host publicationBioluminescence Imaging
PublisherElsevier
Pages245-256
Number of pages12
Volume4
ISBN (Print)978-0-444-53633-4
Publication statusPublished - 2014

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