CRBP-I in the renal tubulointerstitial compartment of healthy rats and rats with renal fibrosis

Katrien Van Beneden, Leonardus Van Grunsven, Caroline Geers, Marina Pauwels, Alexis Desmoulière, Dierik Verbeelen, Albert Geerts, Christiane Van Den Branden

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background. Cellular retinol-binding protein I (CRBP-I), a member of the intracellular lipid-binding protein (iLBP) superfamily, is a specific marker of quiescent stellate cells in the healthy human liver. In the diseased fibrotic/cirrhotic liver, portal and septal myofibroblasts acquire CRBP-I expression, while activated hepatic stellate cells maintain their CRBP-I expression. Here, we investigate the distribution of CRBP-I in the renal cortex of healthy rats and rats with renal fibrosis.
Methods. Kidneys of healthy and adriamycin-treated rats were studied by immunohistochemistry, using antibodies against CRBP-I, desmin, vimentin and alpha-smooth muscle actin (alpha-SMA). Double stainings were done with immunofluorescence. Western blotting was performed to semi-quantify the expression levels of vimentin, desmin, alpha-SMA and CRBP-I.
Results. In the normal rat kidney, the convoluted proximal tubular epithelial cells express CRBP-I; no expression is found in the interstitium, nor in the glomeruli. In the adriamycin-induced fibrotic rat kidney, CRBP-I expression diminishes in the convoluted proximal tubular epithelial cells, whereas peritubular myofibroblasts in the interstitium acquire CRBP-I expression.
Conclusions. In the tubulointerstitial compartment of the adriamycin-induced fibrotic rat kidney, CRBP-I is expressed in a different pattern than in the healthy rat kidney. As the convoluted proximal tubular epithelial cells dedifferentiate during fibrosis, CRBP-I expression decreases. Furthermore, de novo expression of CRBP-I is found in activated myofibroblast-like cells in the interstitium of adriamycin-treated rats. CRBP-I is therefore a useful marker to identify a subpopulation of activated/myodifferentiated fibroblasts in the rat kidney.
Original languageEnglish
Pages (from-to)3464-3471
Number of pages8
JournalNephrology Dialysis Transplantation
Volume23
Publication statusPublished - 2008

Keywords

  • CRBP-I
  • dedifferentiation
  • myofibroblast
  • renal fibrosis
  • tubulointerstitium

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