Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing

Yoni Baert; Jaime Onofre; Dorien Van Saen; Ellen Goossens

doi:10.1007/978-1-4939-7698-0_20

Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing

Yoni Baert, Jaime Onofre, Dorien Van Saen, Ellen Goossens

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Research

10 Citations (Scopus)

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Abstract

Tissue cryopreservation uses very low temperatures to preserve structurally intact living cells in their natural microenvironment. Cell survival is strongly influenced by the biophysical effects of ice during both the freezing and the subsequent thawing. These effects can be controlled by optimizing the fragment size, type of cryoprotectant, and cooling rate. The challenge is to determine cryopreservation parameters that suit all cell types present in the tissue. Here we describe a quick and convenient protocol for the cryopreservation of testicular tissue using an isopropyl-insulated freezing device, which was validated in both a mouse and a human model.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	287-294
Number of pages	8
Volume	1748
ISBN (Electronic)	1940-6029
DOIs	https://doi.org/10.1007/978-1-4939-7698-0_20
Publication status	Published - 20 Feb 2018

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
ISSN (Print)	1064-3745

Keywords

Cryopreservation
Human
Isopropyl
Mouse
Slow freezing
Testicular tissue

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10.1007/978-1-4939-7698-0_20

36425350Accepted author manuscript, 621 KBLicence: Other

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title = "Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing",

abstract = "Tissue cryopreservation uses very low temperatures to preserve structurally intact living cells in their natural microenvironment. Cell survival is strongly influenced by the biophysical effects of ice during both the freezing and the subsequent thawing. These effects can be controlled by optimizing the fragment size, type of cryoprotectant, and cooling rate. The challenge is to determine cryopreservation parameters that suit all cell types present in the tissue. Here we describe a quick and convenient protocol for the cryopreservation of testicular tissue using an isopropyl-insulated freezing device, which was validated in both a mouse and a human model.",

keywords = "Cryopreservation, Human, Isopropyl, Mouse, Slow freezing, Testicular tissue",

author = "Yoni Baert and Jaime Onofre and {Van Saen}, Dorien and Ellen Goossens",

year = "2018",

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doi = "10.1007/978-1-4939-7698-0_20",

language = "English",

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TY - CHAP

T1 - Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing

AU - Baert, Yoni

AU - Onofre, Jaime

AU - Van Saen, Dorien

AU - Goossens, Ellen

PY - 2018/2/20

Y1 - 2018/2/20

N2 - Tissue cryopreservation uses very low temperatures to preserve structurally intact living cells in their natural microenvironment. Cell survival is strongly influenced by the biophysical effects of ice during both the freezing and the subsequent thawing. These effects can be controlled by optimizing the fragment size, type of cryoprotectant, and cooling rate. The challenge is to determine cryopreservation parameters that suit all cell types present in the tissue. Here we describe a quick and convenient protocol for the cryopreservation of testicular tissue using an isopropyl-insulated freezing device, which was validated in both a mouse and a human model.

AB - Tissue cryopreservation uses very low temperatures to preserve structurally intact living cells in their natural microenvironment. Cell survival is strongly influenced by the biophysical effects of ice during both the freezing and the subsequent thawing. These effects can be controlled by optimizing the fragment size, type of cryoprotectant, and cooling rate. The challenge is to determine cryopreservation parameters that suit all cell types present in the tissue. Here we describe a quick and convenient protocol for the cryopreservation of testicular tissue using an isopropyl-insulated freezing device, which was validated in both a mouse and a human model.

KW - Cryopreservation

KW - Human

KW - Isopropyl

KW - Mouse

KW - Slow freezing

KW - Testicular tissue

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U2 - 10.1007/978-1-4939-7698-0_20

DO - 10.1007/978-1-4939-7698-0_20

M3 - Chapter

C2 - 29453579

SN - 1064-3745

VL - 1748

T3 - Methods in Molecular Biology

SP - 287

EP - 294

BT - Methods in Molecular Biology

ER -

Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing

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Spermatogonial stem cell preservation and restoration.

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Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing

Abstract

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Keywords

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Spermatogonial stem cell preservation and restoration.

Cite this