Crystallization of the Staphylococcus aureus MazF mRNA interferase

Valentina Zorzini; Sarah Haesaerts; Niles Donegan; Zhibiao Fu; Ambrose Cheung; Nico Van Nuland; Remy Loris

Crystallization of the Staphylococcus aureus MazF mRNA interferase

Valentina Zorzini, Sarah Haesaerts, Niles Donegan, Zhibiao Fu, Ambrose Cheung, Nico Van Nuland, Remy Loris

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

mazEF modules encode toxin-antitoxin pairs that are involved in the bacterial stress response through controlled and specific degradation of mRNA. Staphylococcus aureus MazF and MazE constitute a unique toxin-antitoxin module under regulation of the sigB operon. A MazF-type mRNA interferase is combined with an antitoxin of unknown fold. Crystals of S. aureus MazF (SaMazF) were grown in space group P2(1)2(1)2(1). The crystals diffracted to 2.1 A resolution and are likely to contain two SaMazF dimers in the asymmetric unit.

Original language	English
Pages (from-to)	386-389
Number of pages	4
Journal	Acta Crystallogr F Struct Biol Commun
Volume	67
Publication status	Published - 2011

Keywords

toxin-antitoxin
structural biology
macromolecular crystallography
ribonuclease
mRNA interferase

AII57: Outsourcing naar VUB binnen samenwerking met Prof. Ambrose Cheung.
Loris, R.
6/11/09 → 31/12/10
Project: Fundamental

1 Membership of external research organisation
1 Talk or presentation at a conference
1 Talk or presentation at a workshop/seminar

VIB Group Leader meeting
Remy Loris (Keynote speaker)
27 Jan 2012
Activity: Talk or presentation › Talk or presentation at a workshop/seminar
International Union of Crystallography (IUCr) Congress and general Assembly 2011
Remy Loris (Keynote speaker)
22 Aug 2011 → 30 Aug 2011
Activity: Talk or presentation › Talk or presentation at a conference
Unknown (External organisation)
Nico Van Nuland (Member)
1 Jan 2009 → 1 Nov 2013
Activity: Membership › Membership of external research organisation

Cite this

@article{16e95a2467f14cae80908786afe0a328,

title = "Crystallization of the Staphylococcus aureus MazF mRNA interferase",

abstract = "mazEF modules encode toxin-antitoxin pairs that are involved in the bacterial stress response through controlled and specific degradation of mRNA. Staphylococcus aureus MazF and MazE constitute a unique toxin-antitoxin module under regulation of the sigB operon. A MazF-type mRNA interferase is combined with an antitoxin of unknown fold. Crystals of S. aureus MazF (SaMazF) were grown in space group P2(1)2(1)2(1). The crystals diffracted to 2.1 A resolution and are likely to contain two SaMazF dimers in the asymmetric unit.",

keywords = "toxin-antitoxin, structural biology, macromolecular crystallography, ribonuclease, mRNA interferase",

author = "Valentina Zorzini and Sarah Haesaerts and Niles Donegan and Zhibiao Fu and Ambrose Cheung and {Van Nuland}, Nico and Remy Loris",

year = "2011",

language = "English",

volume = "67",

pages = "386--389",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

issn = "1744-3091",

publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Crystallization of the Staphylococcus aureus MazF mRNA interferase

AU - Zorzini, Valentina

AU - Haesaerts, Sarah

AU - Donegan, Niles

AU - Fu, Zhibiao

AU - Cheung, Ambrose

AU - Van Nuland, Nico

AU - Loris, Remy

PY - 2011

Y1 - 2011

N2 - mazEF modules encode toxin-antitoxin pairs that are involved in the bacterial stress response through controlled and specific degradation of mRNA. Staphylococcus aureus MazF and MazE constitute a unique toxin-antitoxin module under regulation of the sigB operon. A MazF-type mRNA interferase is combined with an antitoxin of unknown fold. Crystals of S. aureus MazF (SaMazF) were grown in space group P2(1)2(1)2(1). The crystals diffracted to 2.1 A resolution and are likely to contain two SaMazF dimers in the asymmetric unit.

AB - mazEF modules encode toxin-antitoxin pairs that are involved in the bacterial stress response through controlled and specific degradation of mRNA. Staphylococcus aureus MazF and MazE constitute a unique toxin-antitoxin module under regulation of the sigB operon. A MazF-type mRNA interferase is combined with an antitoxin of unknown fold. Crystals of S. aureus MazF (SaMazF) were grown in space group P2(1)2(1)2(1). The crystals diffracted to 2.1 A resolution and are likely to contain two SaMazF dimers in the asymmetric unit.

KW - toxin-antitoxin

KW - structural biology

KW - macromolecular crystallography

KW - ribonuclease

KW - mRNA interferase

M3 - Article

VL - 67

SP - 386

EP - 389

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

SN - 1744-3091

ER -

Crystallization of the Staphylococcus aureus MazF mRNA interferase

Abstract

Keywords

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Projects

AII57: Outsourcing naar VUB binnen samenwerking met Prof. Ambrose Cheung.

Activities

VIB Group Leader meeting

International Union of Crystallography (IUCr) Congress and general Assembly 2011

Unknown (External organisation)

Cite this