Custom lifetime phantoms for characterization and benchmarking of a new CAPS fluorescence-lifetime camera

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Fluorescence imaging has been used for quite some time in microscopy, preclinical and medical imaging, as well as in other domains. There has been interest in using the time-behaviour of fluorophores to gain additional information. This time-behaviour, called the fluorescence lifetime, is a property of the fluorophore and can also reveal information about its environment through modulation of this lifetime. Fluorescence-lifetime imaging could improve imaging of existing fluorescent contrast agents or could enable novel applications. The tauCAM, based on the Current-assisted photonic sampler (CAPS), is being developed at our research department with the purpose of achieving real-time fluorescence lifetime imaging. Previous versions have already shown some results in doing lifetime imaging through experiments and demonstrations. Elaborate characterisation of its imaging capabilities in this domain has yet to be performed due to the lack of standardisation and phantoms available for lifetime imaging. In this publication we will discuss the (real-time) lifetime and intensity imaging capabilities of the tauCAM, the latest iteration of our CAPS camera, housing a new 64×64-pixel sensor. The groundwork will be laid for the development of phantoms for lifetime imaging based on phantoms made for fluorescence intensity imaging. These will in turn be measured using reference equipment and used to characterize the accuracy and precision of the results from the tauCAM.
Original languageEnglish
Title of host publicationAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XX
EditorsCaroline Boudoux, James W. Tunnell
Place of PublicationCalifornia, USA
Number of pages13
ISBN (Electronic)9781510647695
ISBN (Print)978-1-5106-4769-5
Publication statusPublished - 7 Mar 2022
EventSPIE Photonics West - San Francisco, United States
Duration: 22 Jan 202227 Jan 2022

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceSPIE Photonics West
Country/TerritoryUnited States
CitySan Francisco


  • fluorescence lifetime
  • Phantoms, imaging
  • Medical Imaging
  • CAPS


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