Design and development of microfluidic chip technology for high-resolution LC separations

Sam Wouters, Sebastiaan Eeltink

Research output: Chapter in Book/Report/Conference proceedingMeeting abstract (Book)

Abstract

The idea of microfluidic systems stems from chips and electronic circuits used in computers. Modern microfluidic chip technology for chemical analysis can be traced back to the early 1990’s when a silica-based chip for gas chromatography was introduced. Microfluidic separation devices for chromatographic analysis offer significant advantages over conventional systems. The main drivers for the development of miniaturized separations systems include (i) the analysis of minute amounts of sample (important for clinical diagnostics), (ii) reduction of waste streams, and (ii) integration of multiple system functionalities, and hence reducing extra-column band-broadening effects.
In the present study, the design aspects and prototyping of integrated microfluidic chips for liquid chromatographic separations is discussed. A chip-based analyzer is being constructed for the analysis of ions with each module featuring its own channel layout and functionality. The chip integrates a separation channel, membrane suppressor technology, and flow-through electrodes for on-chip conductivity detection. The development of a microfluidic device for spatial three-dimensional LC holds the promise provide ultra-high peak capacities. Furthermore, due to parallel developments of all fractions in the second- and third-dimension separations, the analysis time is greatly reduced compared to conventional column-coupled multi-dimensional LC approaches. The different design aspects to create spatial multi-dimensional chip technology will be discussed, which includes the use of physical barriers and 2D and 3D flow distributors to achieve flow control, and implementation of polymer-monolithic stationary phases. Finally, the separation performance, in terms of peak capacity and peak-production rate will be put in perspective with conventional 1D-LC and two-dimensional LC approaches.
Original languageEnglish
Title of host publication44th International Symposium on High Performance Liquid Phase Separations & Related Techniques (HPLC2016)
PublisherHPLC2016
Publication statusPublished - Jun 2016
Event44th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2016) - Marriott San Francisco Marquis, San Francisco, United States
Duration: 19 Jun 201624 Jun 2016
http://www.hplc2016.org/

Conference

Conference44th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2016)
Abbreviated titleHPLC 2016
CountryUnited States
CitySan Francisco
Period19/06/1624/06/16
Internet address

Keywords

  • microfluidics
  • chips
  • LC

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