Design and Validation of Site-Specifically Labeled Single-Domain Antibody-Based Tracers for in Vivo Fluorescence Imaging and Image-Guided Surgery

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Near-infrared fluorescence molecular imaging has become an established preclinical technique to investigate molecular processes in vivo and to study novel therapies. Furthermore, fluorescence molecular imaging is gaining significant interest from clinicians as an intra-operative guidance tool. This technique makes use of targeted fluorescent tracers as contrast agents that recognize specific biomarkers expressed at the site of disease. Single-domain antibodies have shown to possess excellent properties for in vivo imaging in comparison to conventional antibodies. In this chapter, we describe a method for site-specific conjugation of a near-infrared fluorophore to single-domain antibodies by exploiting cysteine-maleimide chemistry. As opposed to random conjugation, site-specific conjugation results in a homogenously labeled fluorescent tracer and avoids inference with antigen binding.

Original languageEnglish
Title of host publicationDesign and Validation of Site-Specifically Labeled Single-Domain Antibody-Based Tracers for in Vivo Fluorescence Imaging and Image-Guided Surgery
PublisherSpringer
Pages395-407
Number of pages13
Volume2446
ISBN (Electronic)1064-3745
ISBN (Print)1940-6029
DOIs
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2446
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

  • Cell Line, Tumor
  • Fluorescent Dyes/chemistry
  • Molecular Imaging/methods
  • Optical Imaging/methods
  • Single-Domain Antibodies/chemistry
  • Surgery, Computer-Assisted

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