Design of a microfluidic device for spatial two-dimensional LC separations

Bert Wouters, Jelle De Vos, Gert Desmet, P.j. Schoenmakers, Sebastiaan Eeltink

Research output: Unpublished contribution to conferenceUnpublished paper

Abstract

A polymer-based microfluidic device has been designed and constructed for comprehensive spatial two-dimensional chromatography, with each peak being characterized by its coordinates in the plane. The device manufactured from cyclic olefin copolymer (COC) features a first-dimension (1D) separation channel and 21 parallel second-dimension (2D) separation channels orientated perpendicular to the 1D channel. Compartmentalization of 1D and 2D flow by a physical barrier allowed for a preferential mobile phase flow path mainly restricted to the 1D separation channel, with a minimal dispersion into the 2D separation channels. After a 1D separation was halted, a radially-interconnected flow distributor of diamond-shaped pillars with diverging side-walls introduced the mobile phase for the 2D separation, allowing eluent inside the 1D channel to be fractionated and simultaneously injected into the 2D channels. A hydrophobic methacrylate-based monolithic stationary phase was polymerized in-situ in desired locations via a UV-initiated polymerization reaction employing a photo mask.
Original languageEnglish
Publication statusPublished - 4 Sep 2013
Eventthe 9th Balaton Symposium on High-Performance Separation Methods - Siofok, Hungary
Duration: 4 Sep 20136 Sep 2013

Conference

Conferencethe 9th Balaton Symposium on High-Performance Separation Methods
CountryHungary
CitySiofok
Period4/09/136/09/13

Keywords

  • spatial chromatography
  • flow distributor
  • monolith

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