Development and optimization of separation methods for viral compounds.

Iuliana Oita

Research output: ThesisPhD Thesis

Abstract

Viruses are widespread and ecologically important organisms mostly known for their small size and ability to cause diseases. Since their discovery in the late 19th century, viruses were constantly in the spotlight for their pathogenic properties, but over the last years they became an inspiration source also for architects, nanotechnologists, pharmacologists, or material science professionals. Separation methods, such as CE or HPLC, are fast, reproducible, sensible and accurate techniques. Compared to traditional molecular biology tools, separation methods are able to determine both virus concentration and sample purity within one run, while the infective properties of the virus particles are not mandatory. Still, the traditional molecular biology tools have an important advantage when specificity and, sometimes, sensitivity are considered. However, the advantage of the molecular biology tools over separation methods might decrease in the future, as better analytical equipments will be available in response to the technological advancement. The aim of this thesis was to evaluate the potential of separation-science methods in virology. As a consequence, better sample characterization and further insights into unsolved virus mysteries might be obtained.
Original languageEnglish
Awarding Institution
  • Vrije Universiteit Brussel
Supervisors/Advisors
  • Dejaegher, Bieke, Co-Supervisor
  • Thys, Bert, Co-Supervisor
  • Rombaut, Bartholomeus, Supervisor
Place of PublicationBrussels
Publication statusPublished - 2012

Keywords

  • Viruses

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