Development of a capillary electrophoresis method for the separation of flavonolignans in silymarin complex

Petra Riasová, Jaroslav Jenčo, David Moreno-González, Yvan Vander Heyden, Debby Mangelings, Miroslav Polášek, Pavel Jáč

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
19 Downloads (Pure)


CE method for the baseline separation of structurally similar flavonolignans silybin A, silybin B, isosilybin A, isosilybin B, silychristin, silydianin, and their precursor taxifolin in silymarin complex has been developed and validated. The optimized background electrolyte was 100 mmol/L boric acid (pH 9.0) containing 5 mmol/L heptakis(2,3,6-tri-O-methyl)-β-CD and 10% (v/v) of methanol. The separation was carried out in an 80.5/72 cm (50 μm id) fused silica capillary at +25 kV with UV detection at 200 nm. Genistein (10 μg/mL) was used as internal standard. The resolution between the diastereomers of silybin and isosilybin was 1.73 and 2.59, respectively. The method was validated for each analyte in a concentration range of 2.5-50 μg/mL. The calibration curves were rectilinear with correlation coefficients ≥0.9972. The method was applied to determine flavonolignans in two dietary supplements containing Silybum marianum extract. The accuracy was evaluated by comparing the results of the CE analyses of the dietary supplements with those of the reference United States Pharmacopeial HPLC method. The unpaired t-test did not show a statistically significant difference between the results of both the proposed CE and the reference method (p > 0.05, n = 3).

Original languageEnglish
Pages (from-to)930-938
Number of pages9
Issue number9-10
Early online date9 Nov 2021
Publication statusPublished - May 2022

Bibliographical note

© 2021 Wiley-VCH GmbH.


  • CE
  • Dietary supplements
  • Flavonolignans
  • Heptakis(2,3, 6-tri-O-methyl)-β-CD
  • Silymarin


Dive into the research topics of 'Development of a capillary electrophoresis method for the separation of flavonolignans in silymarin complex'. Together they form a unique fingerprint.

Cite this