Development of a generic liquid chromatography method for the analysis of residual multi-class antibiotics in food products at 1500 bar

Research output: Unpublished contribution to conferencePoster

Abstract

Peak capacity (Nc) in gradient separation is the primary metric of separation performance. Increasing column length improves gradient peak capacity but incurs additional pressure drop on the system (Figure 1). Next generation instrumentation are designed to withstand higher backpressures to capitalize on this superior performance.
Kinetic plots allow the prediction of resulting peak capacities when operating under a set pressure constrained “kinetic limit”. Kinetic plot modeling can be particularly useful for trace impurity assays to ensure quantification of co-eluting compounds in the lowest analysis time.
Herein, we provide a kinetic plot-based model to elucidate the expected peak capacities of a range of broad-spectrum antibiotics achievable with commercially available core-shell columns. Following, we extrapolate the peak capacities to the kinetic limit of 1500 bar to predict the flow rates and corresponding column lengths to achieve certain peak capacity thresholds which we confirm in practice with coupled column experiments up to 400 cm.

Original languageEnglish
Publication statusPublished - 22 Jul 2022
Event50th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2022) - San Diego, United States
Duration: 18 Jun 202223 Jun 2022
https://hplc2022.org/

Conference

Conference50th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2022)
Abbreviated titleHPLC 2022
Country/TerritoryUnited States
CitySan Diego
Period18/06/2223/06/22
Internet address

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