Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections

Joar E Pinto Torres, Julie Goossens, Jianzu Ding, Zeng Li, Shaohong Lu, Didier Vertommen, Peter Naniima, Rui Chen, Serge Muyldermans, Yann G-J Sterckx, Stefan Magez

Research output: Contribution to journalArticle

27 Citations (Scopus)


Animal African trypanosomosis (AAT), a disease affecting livestock, is caused by parasites of the Trypanosoma genus (mainly T. vivax and T. congolense). AAT is widespread in Sub-Saharan Africa, where it continues to impose a heavy socio-economic burden as it renders development of sustainable livestock rearing very strenuous. Active case-finding and the identification of infected animals prior to initiation of drug treatment requires the availability of sensitive and specific diagnostic tests. In this paper, we describe the development of two heterologous sandwich assay formats (ELISA and LFA) for T. congolense detection through the use of Nanobodies (Nbs). The immunisation of an alpaca with a secretome mix from two T. congolense strains resulted in the identification of a Nb pair (Nb44/Nb42) that specifically targets the glycolytic enzyme pyruvate kinase. We demonstrate that the Nb44/Nb42 ELISA and LFA can be employed to detect parasitaemia in plasma samples from experimentally infected mice and cattle and, additionally, that they can serve as 'test-of-cure' tools. Altogether, the findings in this paper present the development and evaluation of the first Nb-based antigen detection LFA to identify active T. congolense infections.

Original languageEnglish
Article number9019
Number of pages15
JournalScientific Reports - Nature
Issue number1
Publication statusPublished - 13 Jun 2018


  • Journal Article


Dive into the research topics of 'Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections'. Together they form a unique fingerprint.

Cite this