Differences in collagen expression in cumulus cells induced by LH-activity of highly purified menotrophin in comparison to recombinant FSH in a follicle culture model

Tom Adriaenssens, Ingrid Segers, Claire Mazoyer, Sandra Wathlet, Joan-Carles Arce, Johan Smitz

Research output: Chapter in Book/Report/Conference proceedingConference paper

Abstract

Objective
To understand the molecular mechanisms underlying the favourable effects of LH activity in ART on oocyte and embryo quality. Micro array analysis in mouse and human cumulus-corona cells (CC) indicated that Col4a1/2 and Col6a2 are upregulated in preovulatory follicles when stimulated with FSH alone compared to FSH plus LH-activity. Col4 expression is related to progesterone production and Col6 interacts with hyaluronic acid, an important extracellular matrix (ECM) constituent.

Design
Early preantral mouse follicles were individually grown in 10IU/L recombinant FSH (GONAL-F, Serono) for 12 days, or in a superovulation condition where follicles received from day 6 to day 12 (antral phase) either 100IU/L recombinant FSH or 100IU/L highly purified menotrophin (MENOPUR, Ferring Pharmaceuticals). CC cells were assayed on day 9 (early antral follicle) and day 12 (preovulatory follicle) for the amounts of Col4a1/2 and 6a2 mRNA.
Original languageEnglish
Title of host publicationFertility and Sterility
Pages170-171
Number of pages2
Volume88
Publication statusPublished - 2007
EventFinds and Results from the Swedish Cyprus Expedition: A Gender Perspective at the Medelhavsmuseet - Stockholm, Sweden
Duration: 21 Sep 200925 Sep 2009

Conference

ConferenceFinds and Results from the Swedish Cyprus Expedition: A Gender Perspective at the Medelhavsmuseet
Country/TerritorySweden
CityStockholm
Period21/09/0925/09/09

Keywords

  • LH
  • FSH
  • follicle culture

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