Direct detection and identification of African trypanosomes by fluorescence in situ hybridization with peptide nucleic acid probes

M Radwanska, S Magez, H Perry-O'Keefe, H Stender, J Coull, J M Sternberg, P Büscher, J J Hyldig-Nielsen

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We have developed a rapid and easy to perform fluorescence in situ hybridization test that allows specific identification of trypanosomes from the subgenus Trypanozoon, using peptide nucleic acid probes. Probes were designed to target subgenus-specific sequences on the multiple-copy 18S rRNA, greatly facilitating the detection of a single trypanosome.

Original languageEnglish
Pages (from-to)4295-4297
Number of pages3
JournalJournal of Clinical Microbiology
Volume40
Issue number11
DOIs
Publication statusPublished - Nov 2002

Keywords

  • Animals
  • Blood
  • Cattle
  • DNA, Ribosomal
  • Humans
  • In Situ Hybridization, Fluorescence
  • Nucleic Acid Probes
  • Peptide Nucleic Acids
  • RNA, Ribosomal, 18S
  • Trypanosoma
  • Trypanosomiasis, African

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