Dogs as potential mechanical vector of transmission of Brucella. Spp., in dairy cattle in the sierra of Ecuador

Francisco Benitez Capistros, Jorge Ron Roman, Pablo González Andrade, Elizabeth Minda Aluisa, Washington Benítez Ortiz, Dirk Berkvens (Editor), Claude Saegerman (Editor)

Research output: Chapter in Book/Report/Conference proceedingConference paper

Abstract

In 2008 a study in cattle farms in Ecuador was set up to determine whether dogs harboured strains of Brucella spp., endemic at the region. Serum samples from dogs (n=151) living on farms (n=34) in Mejia canton in Pichincha province, Ecuador, were tested for antibodies against smooth Brucella Spp., by three immunodiagnostic assays. Highly positive dogs (n=5) were euthanized in order to cultured samples of lymph nodes and organs. From a dairy farm with positive dogs, sera from cows (n=93) were analysed by two immunodiagnostic assays and milk samples from seropositive cows (n=7) were cultured. In addition, isolates were investigated by PCR-711.

85.43% of the dogs had contact with cattle and drank raw milk, 64.24% regularly ate aborted material. In total 43 dogs (30.46%) reacted positive at least to one serodiagnostic test; individual tests showed 15.89% positive reactions when tested by the Rose Bengal test (RB), 11.92% by the Wright's Slow Agglutination Test" (SAT-EDTA) and 23.85% by the indirect Enzyme Linked ImmunoSorbent Assay (iELISA). Only 13 (8.60%) dogs tested positive in all three tests. Four bovines (4.30%) were positive on RB and SAT-EDTA. Brucella spp. was identified in isolations from dogs (n=5) and bovines (n=1), by routine biochemical assays and PCR-711.

Our results demonstrate that control of brucellosis should not be restricted to bovines, for which economic losses are obvious. Equal attention should be given to other animals, among them dogs living on farms or in the neighbourhood of farms, as potential mechanical vector of transmission of this zoonosis
Original languageEnglish
Title of host publicationColloquium on Zoonoses and Neglected Infectious Diseases of Africa. Programme and abstract information.
PublisherInstitute of Tropical Medicine (ITM), Department of Veterinary Tropical Diseases of the University of Pretoria (DVTD). Johannesburg, South Africa
Pages41-41
Number of pages1
Publication statusPublished - 1 Nov 2011
EventUnknown -
Duration: 1 Nov 2011 → …

Publication series

NameColloquium on Zoonoses and Neglected Infectious Diseases of Africa. Programme and abstract information.

Conference

ConferenceUnknown
Period1/11/11 → …

Keywords

  • Brucellosis
  • Epidemiology
  • Immunodiagnostic assays
  • Mechanical vector of transmission
  • Zoonoses

Fingerprint

Dive into the research topics of 'Dogs as potential mechanical vector of transmission of Brucella. Spp., in dairy cattle in the sierra of Ecuador'. Together they form a unique fingerprint.

Cite this