Effect of post-ischaemic mild hypothermia on apoptosis and inflammation after transient focal cerebral ischaemia in the Endothelin-1 rat model

An-Gaelle Ceulemans, An Van Hemelrijck, Sophie Sarre, Said Hachimi-Idrissi, Yvette Michotte

Research output: Chapter in Book/Report/Conference proceedingMeeting abstract (Book)

Abstract

The endothelin-1 (Et-1) rat model, in which Et-1 is infused adjacent to the middle cerebral artery to induce a transient focal cerebral ischaemia, is used to study the effects of post-ischaemic mild hypothermia, which has proven to be a robust neuroprotectant in experimental stroke models. In this model, the core and the penumbra are respectively represented by the striatum and the cortex. Anaesthetized rats are kept either under normothermic (37°C) or post-ischaemic mild hypothermic (34°C for 2 h) conditions. In previous research in our laboratory, the volume of ischaemic damage and the degree of apoptosis 24 h after the insult correlated well. Hypothermia reduced apoptosis and infarct size in the cortex, but no effect was seen in the striatum. The aim of the present project is to study apoptosis also after 72 h and one week. Similarly, we will determine pro- and anti-inflammatory markers, such as interleukin-1 beta, tumor necrosis factor alpha and transforming growth factor beta at these time points. Motor impairment will be evaluated using the cylinder test and neurological deficit score. Our final objective is to determine the optimal time of onset and duration of hypothermia in the Et-1 rat model for transient focal cerebral ischaemia.
Original languageEnglish
Title of host publicationProgram and Abstract Book of the 7th Bi-annual Meeting of the "Belgian Society for Neuroscience"
Pages35
Number of pages1
Publication statusPublished - 7 May 2007

Publication series

NameProgram and Abstract Book of the 7th Bi-annual Meeting of the "Belgian Society for Neuroscience"

Keywords

  • stroke
  • hypothermia
  • inflammation
  • endothelin-1

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