Emergence of livestock-associated MRSA isolated from cystic fibrosis patients: Result of a Belgian national survey

M Dodémont; M A Argudín; J Willekens; E Vanderhelst; D Pierard; V Y Miendje Deyi; L Hanssens; H Franckx; P Schelstraete; I Leroux-Roels; C Nonhoff; A Deplano; C Knoop; A Malfroot; O Denis

doi:10.1016/j.jcf.2018.04.008

Emergence of livestock-associated MRSA isolated from cystic fibrosis patients: Result of a Belgian national survey

M Dodémont, M A Argudín, J Willekens, E Vanderhelst, D Pierard, V Y Miendje Deyi, L Hanssens, H Franckx, P Schelstraete, I Leroux-Roels, C Nonhoff, A Deplano, C Knoop, A Malfroot, O Denis

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Abstract

BACKGROUND: This study aims to determine the prevalence and characteristics of Staphylococcus aureus in Belgian cystic fibrosis (CF) patients.

METHODS: Non-duplicate respiratory samples from 510 CF-patients (2012-2013) were examined. One isolate per patient was analysed unless different phenotypes were recovered. Isolates were investigated for mecA/mecC, toxins presence, spa-typing, MLST and SCCmec-typing. Potential livestock-associated (LA) isolates were examined for their immune-evasion-cluster (IEC) genes.

RESULTS: S. aureus (n = 380), including 41 small-colony variants (SCVs), were isolated from 66.7% patients. The prevalence of methicillin-resistant S. aureus (MRSA) colonization was 4.9%. Two MRSA isolates carried toxic shock syndrome toxin 1 (TSST-1). Most MRSA (65%) belonged to two nosocomial epidemic clones (CC5, CC8) widespread in Belgium. Methicillin susceptible S. aureus (MSSA) showed great genetic diversity. Five of 33 isolates belonging to potential LA-lineages were IEC negative, including three methicillin-resistant isolates, suggesting an animal origin.

CONCLUSIONS: The MRSA-prevalence in Belgian CF-patients remained constant (2001-2013), but SCV-prevalence increased. Most MRSA belonged to health-care-associated clones. Three patients carrying LA-MRSA were found, requiring further investigation to determine the risk factors for LA-MRSA acquisition.

Original language	English
Pages (from-to)	86-93
Number of pages	8
Journal	Journal of Cystic Fibrosis
Volume	18
Issue number	1
Early online date	24 May 2018
DOIs	https://doi.org/10.1016/j.jcf.2018.04.008
Publication status	Published - Jan 2019

Keywords

CC398
LA-MRSA
Small colony variant
spa-typing

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Dodémont, M., Argudín, M. A., Willekens, J., Vanderhelst, E., Pierard, D., Miendje Deyi, V. Y., Hanssens, L., Franckx, H., Schelstraete, P., Leroux-Roels, I., Nonhoff, C., Deplano, A., Knoop, C., Malfroot, A., & Denis, O. (2019). Emergence of livestock-associated MRSA isolated from cystic fibrosis patients: Result of a Belgian national survey. Journal of Cystic Fibrosis, 18(1), 86-93. https://doi.org/10.1016/j.jcf.2018.04.008

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title = "Emergence of livestock-associated MRSA isolated from cystic fibrosis patients: Result of a Belgian national survey",

abstract = "BACKGROUND: This study aims to determine the prevalence and characteristics of Staphylococcus aureus in Belgian cystic fibrosis (CF) patients.METHODS: Non-duplicate respiratory samples from 510 CF-patients (2012-2013) were examined. One isolate per patient was analysed unless different phenotypes were recovered. Isolates were investigated for mecA/mecC, toxins presence, spa-typing, MLST and SCCmec-typing. Potential livestock-associated (LA) isolates were examined for their immune-evasion-cluster (IEC) genes.RESULTS: S. aureus (n = 380), including 41 small-colony variants (SCVs), were isolated from 66.7% patients. The prevalence of methicillin-resistant S. aureus (MRSA) colonization was 4.9%. Two MRSA isolates carried toxic shock syndrome toxin 1 (TSST-1). Most MRSA (65%) belonged to two nosocomial epidemic clones (CC5, CC8) widespread in Belgium. Methicillin susceptible S. aureus (MSSA) showed great genetic diversity. Five of 33 isolates belonging to potential LA-lineages were IEC negative, including three methicillin-resistant isolates, suggesting an animal origin.CONCLUSIONS: The MRSA-prevalence in Belgian CF-patients remained constant (2001-2013), but SCV-prevalence increased. Most MRSA belonged to health-care-associated clones. Three patients carrying LA-MRSA were found, requiring further investigation to determine the risk factors for LA-MRSA acquisition.",

keywords = "CC398, LA-MRSA, Small colony variant, spa-typing",

author = "M Dod{\'e}mont and Argud{\'i}n, {M A} and J Willekens and E Vanderhelst and D Pierard and {Miendje Deyi}, {V Y} and L Hanssens and H Franckx and P Schelstraete and I Leroux-Roels and C Nonhoff and A Deplano and C Knoop and A Malfroot and O Denis",

year = "2019",

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Dodémont, M, Argudín, MA, Willekens, J, Vanderhelst, E , Pierard, D, Miendje Deyi, VY, Hanssens, L, Franckx, H, Schelstraete, P, Leroux-Roels, I, Nonhoff, C, Deplano, A, Knoop, C , Malfroot, A & Denis, O 2019, 'Emergence of livestock-associated MRSA isolated from cystic fibrosis patients: Result of a Belgian national survey', Journal of Cystic Fibrosis, vol. 18, no. 1, pp. 86-93. https://doi.org/10.1016/j.jcf.2018.04.008

TY - JOUR

T1 - Emergence of livestock-associated MRSA isolated from cystic fibrosis patients

T2 - Result of a Belgian national survey

AU - Dodémont, M

AU - Argudín, M A

AU - Willekens, J

AU - Vanderhelst, E

AU - Pierard, D

AU - Miendje Deyi, V Y

AU - Hanssens, L

AU - Franckx, H

AU - Schelstraete, P

AU - Leroux-Roels, I

AU - Nonhoff, C

AU - Deplano, A

AU - Knoop, C

AU - Malfroot, A

AU - Denis, O

PY - 2019/1

Y1 - 2019/1

N2 - BACKGROUND: This study aims to determine the prevalence and characteristics of Staphylococcus aureus in Belgian cystic fibrosis (CF) patients.METHODS: Non-duplicate respiratory samples from 510 CF-patients (2012-2013) were examined. One isolate per patient was analysed unless different phenotypes were recovered. Isolates were investigated for mecA/mecC, toxins presence, spa-typing, MLST and SCCmec-typing. Potential livestock-associated (LA) isolates were examined for their immune-evasion-cluster (IEC) genes.RESULTS: S. aureus (n = 380), including 41 small-colony variants (SCVs), were isolated from 66.7% patients. The prevalence of methicillin-resistant S. aureus (MRSA) colonization was 4.9%. Two MRSA isolates carried toxic shock syndrome toxin 1 (TSST-1). Most MRSA (65%) belonged to two nosocomial epidemic clones (CC5, CC8) widespread in Belgium. Methicillin susceptible S. aureus (MSSA) showed great genetic diversity. Five of 33 isolates belonging to potential LA-lineages were IEC negative, including three methicillin-resistant isolates, suggesting an animal origin.CONCLUSIONS: The MRSA-prevalence in Belgian CF-patients remained constant (2001-2013), but SCV-prevalence increased. Most MRSA belonged to health-care-associated clones. Three patients carrying LA-MRSA were found, requiring further investigation to determine the risk factors for LA-MRSA acquisition.

AB - BACKGROUND: This study aims to determine the prevalence and characteristics of Staphylococcus aureus in Belgian cystic fibrosis (CF) patients.METHODS: Non-duplicate respiratory samples from 510 CF-patients (2012-2013) were examined. One isolate per patient was analysed unless different phenotypes were recovered. Isolates were investigated for mecA/mecC, toxins presence, spa-typing, MLST and SCCmec-typing. Potential livestock-associated (LA) isolates were examined for their immune-evasion-cluster (IEC) genes.RESULTS: S. aureus (n = 380), including 41 small-colony variants (SCVs), were isolated from 66.7% patients. The prevalence of methicillin-resistant S. aureus (MRSA) colonization was 4.9%. Two MRSA isolates carried toxic shock syndrome toxin 1 (TSST-1). Most MRSA (65%) belonged to two nosocomial epidemic clones (CC5, CC8) widespread in Belgium. Methicillin susceptible S. aureus (MSSA) showed great genetic diversity. Five of 33 isolates belonging to potential LA-lineages were IEC negative, including three methicillin-resistant isolates, suggesting an animal origin.CONCLUSIONS: The MRSA-prevalence in Belgian CF-patients remained constant (2001-2013), but SCV-prevalence increased. Most MRSA belonged to health-care-associated clones. Three patients carrying LA-MRSA were found, requiring further investigation to determine the risk factors for LA-MRSA acquisition.

KW - CC398

KW - LA-MRSA

KW - Small colony variant

KW - spa-typing

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U2 - 10.1016/j.jcf.2018.04.008

DO - 10.1016/j.jcf.2018.04.008

M3 - Article

C2 - 29805051

SN - 1569-1993

VL - 18

SP - 86

EP - 93

JO - Journal of Cystic Fibrosis

JF - Journal of Cystic Fibrosis

IS - 1

ER -

Emergence of livestock-associated MRSA isolated from cystic fibrosis patients: Result of a Belgian national survey

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Keywords

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