Endocrine activity in hospital, WWTP effluent, and river waters from the Brussels region, Belgium using the BG1Luc4E2 CALUX Bioassay.

Kersten Van Langenhove, Tim Reyns, Tara Vandermarken, Pierre Servais, Michael S. Denison, Joris Van Loco, Marc Elskens

Research output: Unpublished contribution to conferencePoster

Abstract

Endocrine disrupting chemicals (EDCs) are gaining in worldwide attention
due to their omnipresence, wide range in chemical properties and possible
effects on wildlife populations. Problems with these EDCs are their
continuous release into the environment (industrial, domestic, inefficient
removal). For this reason, the Water Framework Directive (amended in
2013/39/EU) established a priority list of 33 new and 8 old pollutants and
also placed 15 compounds (including estrogens) onto a watch list.
Rather than looking solely for concentrations of targeted and well-known
EDCs, this project strives to combine bio-analytical and chemo-analytical
data on the Zenne river crossing Brussels and hospital effluents (a potential
major source for EDCs due to high consumption of pharmaceuticals and
personal care products) for the first time. Bioassays allow scientists to use
in vitro receptor models to assess endocrine activity by comparing the
mixture or cocktail effect originating from the sample extract. Added value
is that this technique picks up effects from compounds that are not
routinely monitored in targeted chemo-analytical methods and can detect
them at low amounts (pg/L EEQ, Estrogen EQuivalence).
Samples are extracted using Oasis HLB SPE columns and analyzed with
human ovarian adenocarcinoma (BG1) cells transfected with a luciferase
reporter gene. Values ranged from 0.77 to 1.85ng EEQ/L for the Zenne river
water, 98ng EEQ/L for hospital effluent and, respectively, 52.3 and 1.2ng
EEQ/L for WWTP influent and effluent. For comparison reasons, commercial
drinking waters amount to 0.004-0.070 ng EEQ/L. These results indicate that
effluent values are high post-discharge of human activities, EDCs are only
partially removed by processes in WWTPs, and that effluents contribute
significantly to EDC loads in the Zenne river.
Original languageEnglish
Pages52
Publication statusPublished - 1 Mar 2016
EventChemCYS 2016 - Blankenberge, Belgium
Duration: 16 Mar 201618 Mar 2016

Conference

ConferenceChemCYS 2016
CountryBelgium
CityBlankenberge
Period16/03/1618/03/16

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