Projects per year
Abstract
oxin-antitoxin (TA) modules are pairs of genes essential for bacterial regulation upon environmental stresses. The mazEF module encodes the MazF toxin and its cognate MazE antitoxin. The highly dynamic MazE possesses an N-terminal DNA binding domain through which it can negatively regulate its own promoter. Despite being one of the first TA systems studied, transcriptional regulation of Escherichia coli mazEF remains poorly understood. This paper presents the solution structure of C-terminal truncated E. coli MazE and a MazE-DNA model with a DNA palindrome sequence about 10 bp upstream of the mazEF promoter. The work has led to a transcription regulator-DNA model, which has remained elusive thus far in the E. coli toxin-antitoxin family. Multiple complementary techniques including NMR, SAXS and ITC show that the long intrinsically disordered C-termini in MazE, required for MazF neutralization, does not affect the interactions between the antitoxin and its operator. Rather, the MazE C-terminus plays an important role in the MazF binding, which was found to increase the MazE affinity for the palindromic single site operator.
Original language | English |
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Pages (from-to) | 1241-1256 |
Number of pages | 16 |
Journal | Nucleic Acids Res |
Volume | 43 |
DOIs | |
Publication status | Published - 2015 |
Keywords
- Bacterial stress response
- Bio-SAXS
- Persister
- Persistence
- Protein-DNA interaction
- Protein-DNA complex
- NMR spectroscopy
Fingerprint
Dive into the research topics of 'Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding'. Together they form a unique fingerprint.Projects
- 6 Finished
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SRP13: Strategic Research Programme: Structure and dynamics of macromolecular complexes in health and disease
Steyaert, J., Remaut, H. K., Loris, R., Efremov, R., Steyaert, J., Loris, R. & Remaut, H. K.
1/11/12 → 31/10/24
Project: Fundamental
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FWOTM615: Regulatory mechanisms within the mazEF family of toxinantitoxin modules
1/10/11 → 30/09/15
Project: Fundamental
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Activities
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Toxin-antitoxin modules as a model for functional disorder in bacteria
Remy Loris (Speaker)
2 Jul 2018 → 6 Jul 2018Activity: Talk or presentation › Talk or presentation at a conference
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Structural dynamics in cellular communication
Alexandra Vandervelde (Participant)
9 Feb 2015 → 10 Feb 2015Activity: Participating in or organising an event › Participation in conference
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Structural dynamics in cellular communication
San Hadzi (Participant)
9 Feb 2015 → 10 Feb 2015Activity: Participating in or organising an event › Participation in conference