Evaluation of system performance in ultra-high-pressure operation mode

Zhuoheng Zhou, Mauro De Pra, Frank Steiner, Gert Desmet, Sebastiaan Eeltink

Research output: Unpublished contribution to conferenceUnpublished paper

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Abstract

The current state-of-the-art UHPLC technology comprises instrumentation allowing to operate columns packed with 1.5 um core-shell particles at operating pressures up to 1500 bar. To effectively utilize the potential of these columns, yielding peak volumes ranging between 0.5 and 10 uL, extra-column dispersion contributions need to be minimized.

In the current study, the instrument configuration of a UHPLC system with 1500 bar pressure capabilities was systematically altered and its effects on separation efficiency and retention were assessed in both the isocratic and gradient modes. Key factors affecting extra-column dispersion were investigated and the optimal system configuration with respect to extra-column dispersion and flow resistance is discussed for isocratic and gradient LC mode. Furthermore, pressure-induced retention effects have been evaluated, which affect method development and HPLC to UHPLC method-transfer strategies.
Original languageEnglish
Pages63-63
Number of pages1
Publication statusPublished - Jan 2020
Event16th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology - Het Pand, Ghent, Belgium
Duration: 29 Jan 202031 Jan 2020
https://kuleuvencongres.be/htc16

Conference

Conference16th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology
Abbreviated titleHTC-16
CountryBelgium
CityGhent
Period29/01/2031/01/20
Internet address

Keywords

  • Ultra-high pressure

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