Evidence of high N 2 fixation rates in the temperate northeast Atlantic

Debany Fonseca-Batista, Xuefeng Li, Virginie Riou, Florian Deman, François Fripiat, Natacha Brion, Marc Elskens, Lei Chou, Frank Dehairs

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15 Citations (Scopus)


Diazotrophic activity and primary production (PP) were investigated along two transects (Belgica BG2014/14 and GEOVIDE cruises) off the western Iberian Margin and the Bay of Biscay in May 2014. Substantial N 2 fixation activity was observed at 8 of the 10 stations sampled, ranging overall from 81 to 384 μmolNm -2 d -1 (0.7 to 8.2 nmolNL -1 d -1 , with two sites close to the Iberian Margin situated between 38.8 and 40.7° N yielding rates reaching up to 1355 and 1533 μmolNm -2 d -1 . Primary production was relatively lower along the Iberian Margin, with rates ranging from 33 to 59 mmol Cm -2 d -1 , while it increased towards the northwest away from the peninsula, reaching as high as 135 mmol Cm -2 d -1 . In agreement with the area-averaged Chl a satellite data contemporaneous with our study period, our results revealed that post-bloom conditions prevailed at most sites, while at the northwesternmost station the bloom was still ongoing. When converted to carbon uptake using Redfield stoichiometry, N 2 fixation could support 1% to 3% of daily PP in the euphotic layer at most sites, except at the two most active sites where this contribution to daily PP could reach up to 25 %. At the two sites where N 2 fixation activity was the highest, the prymnesiophyte.symbiont Candidatus Atelocyanobacterium thalassa (UCYN-A) dominated the nifH sequence pool, while the remaining recovered sequences belonged to non-cyanobacterial phylotypes. At all the other sites, however, the recovered nifH sequences were exclusively assigned phylogenetically to non-cyanobacterial phylotypes. The intense N 2 fixation activities recorded at the time of our study were likely promoted by the availability of phytoplanktonderived organic matter produced during the spring bloom, as evidenced by the significant surface particulate organic carbon concentrations. Also, the presence of excess phosphorus signature in surface waters seemed to contribute to sustaining N 2 fixation, particularly at the sites with extreme activities. These results provide a mechanistic understanding of the unexpectedly high N 2 fixation in productive waters of the temperate North Atlantic and highlight the importance of N 2 fixation for future assessment of the global N inventory.

Original languageEnglish
Pages (from-to)999-1017
Number of pages19
Issue number5
Publication statusPublished - 13 Mar 2019


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