Fabrication of a molecularly imprinted monolithic column via the epitope approach for the selective capillary microextraction of neuropeptides in human plasma

Xiaoyun LEI; Ting Huang; Xiaoping Wu; Debby Mangelings; Ann Van Eeckhaut; Jana Bongaerts; Herman Terryn; Yvan Vander Heyden

doi:10.1016/j.talanta.2022.123397

Fabrication of a molecularly imprinted monolithic column via the epitope approach for the selective capillary microextraction of neuropeptides in human plasma

Xiaoyun LEI, Ting Huang, Xiaoping Wu, Debby Mangelings, Ann Van Eeckhaut, Jana Bongaerts, Herman Terryn, Yvan Vander Heyden

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Abstract

A novel molecularly imprinted monolithic (MIM) column was designed and fabricated using the epitope approach, and was used for the selective capillary microextraction (CME) of the neuropeptides neurotensin (NT) and neuromedin N (NmN). The MIMs were synthesized in a capillary by thermally initiated polymerization of the functional monomer (methacrylic acid (MAA)), in the presence of a dummy template (Pro-Tyr-Ile-Leu (PYIL)), a crosslinker and porogens. The resulting monoliths were characterized by scanning electron microscopy, pore size distribution measurement, and Fourier transform infrared spectroscopy. Different synthesis conditions of the MIM column were investigated. The parameters affecting the MIM-CME performance, including loading, washing and elution protocols, were optimized as well. The MIMs were used to enrich NT and NmN from human plasma prior to HPLC-UV analysis. The imprinted monolith showed an excellent maximum adsorption capacity of 245–711 mg mL−1 and selectivity (imprinting factor of 5.7–13.4) towards its target peptides. Low detection limits of 0.62 and 1.20 nM, and satisfactory recoveries (82.5–98.8%) were obtained for NT and NmN, respectively. The proposed MIM-CME/HPLC-UV method was found suitable to be used as an effective tool for the highly efficient and specific analysis of NT and NmN in human plasma samples.

Original language	English
Article number	123397
Number of pages	9
Journal	Talanta
Volume	243
Early online date	21 Mar 2022
DOIs	https://doi.org/10.1016/j.talanta.2022.123397
Publication status	Published - 1 Jun 2022

Bibliographical note

Funding Information:
The authors are grateful for financial support from the National Natural Science Foundation (Grant No. 22076024 ), and the Major Project of Science and Technology of Fujian Province (Grant No. 2018YZ0002-1 ) of China.

Publisher Copyright:
© 2022 Elsevier B.V.

Copyright:
Copyright 2022 Elsevier B.V., All rights reserved.

Keywords

Neuropeptides
Molecularly imprinted monolithic column
Capillary microextraction
Epitope approach

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title = "Fabrication of a molecularly imprinted monolithic column via the epitope approach for the selective capillary microextraction of neuropeptides in human plasma",

abstract = "A novel molecularly imprinted monolithic (MIM) column was designed and fabricated using the epitope approach, and was used for the selective capillary microextraction (CME) of the neuropeptides neurotensin (NT) and neuromedin N (NmN). The MIMs were synthesized in a capillary by thermally initiated polymerization of the functional monomer (methacrylic acid (MAA)), in the presence of a dummy template (Pro-Tyr-Ile-Leu (PYIL)), a crosslinker and porogens. The resulting monoliths were characterized by scanning electron microscopy, pore size distribution measurement, and Fourier transform infrared spectroscopy. Different synthesis conditions of the MIM column were investigated. The parameters affecting the MIM-CME performance, including loading, washing and elution protocols, were optimized as well. The MIMs were used to enrich NT and NmN from human plasma prior to HPLC-UV analysis. The imprinted monolith showed an excellent maximum adsorption capacity of 245–711 mg mL−1 and selectivity (imprinting factor of 5.7–13.4) towards its target peptides. Low detection limits of 0.62 and 1.20 nM, and satisfactory recoveries (82.5–98.8%) were obtained for NT and NmN, respectively. The proposed MIM-CME/HPLC-UV method was found suitable to be used as an effective tool for the highly efficient and specific analysis of NT and NmN in human plasma samples.",

keywords = "Neuropeptides, Molecularly imprinted monolithic column, Capillary microextraction, Epitope approach",

author = "Xiaoyun LEI and Ting Huang and Xiaoping Wu and Debby Mangelings and {Van Eeckhaut}, Ann and Jana Bongaerts and Herman Terryn and {Vander Heyden}, Yvan",

note = "Funding Information: The authors are grateful for financial support from the National Natural Science Foundation (Grant No. 22076024 ), and the Major Project of Science and Technology of Fujian Province (Grant No. 2018YZ0002-1 ) of China. Publisher Copyright: {\textcopyright} 2022 Elsevier B.V. Copyright: Copyright 2022 Elsevier B.V., All rights reserved.",

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T1 - Fabrication of a molecularly imprinted monolithic column via the epitope approach for the selective capillary microextraction of neuropeptides in human plasma

AU - LEI, Xiaoyun

AU - Huang, Ting

AU - Wu, Xiaoping

AU - Mangelings, Debby

AU - Van Eeckhaut, Ann

AU - Bongaerts, Jana

AU - Terryn, Herman

AU - Vander Heyden, Yvan

N1 - Funding Information: The authors are grateful for financial support from the National Natural Science Foundation (Grant No. 22076024 ), and the Major Project of Science and Technology of Fujian Province (Grant No. 2018YZ0002-1 ) of China. Publisher Copyright: © 2022 Elsevier B.V. Copyright: Copyright 2022 Elsevier B.V., All rights reserved.

PY - 2022/6/1

Y1 - 2022/6/1

N2 - A novel molecularly imprinted monolithic (MIM) column was designed and fabricated using the epitope approach, and was used for the selective capillary microextraction (CME) of the neuropeptides neurotensin (NT) and neuromedin N (NmN). The MIMs were synthesized in a capillary by thermally initiated polymerization of the functional monomer (methacrylic acid (MAA)), in the presence of a dummy template (Pro-Tyr-Ile-Leu (PYIL)), a crosslinker and porogens. The resulting monoliths were characterized by scanning electron microscopy, pore size distribution measurement, and Fourier transform infrared spectroscopy. Different synthesis conditions of the MIM column were investigated. The parameters affecting the MIM-CME performance, including loading, washing and elution protocols, were optimized as well. The MIMs were used to enrich NT and NmN from human plasma prior to HPLC-UV analysis. The imprinted monolith showed an excellent maximum adsorption capacity of 245–711 mg mL−1 and selectivity (imprinting factor of 5.7–13.4) towards its target peptides. Low detection limits of 0.62 and 1.20 nM, and satisfactory recoveries (82.5–98.8%) were obtained for NT and NmN, respectively. The proposed MIM-CME/HPLC-UV method was found suitable to be used as an effective tool for the highly efficient and specific analysis of NT and NmN in human plasma samples.

AB - A novel molecularly imprinted monolithic (MIM) column was designed and fabricated using the epitope approach, and was used for the selective capillary microextraction (CME) of the neuropeptides neurotensin (NT) and neuromedin N (NmN). The MIMs were synthesized in a capillary by thermally initiated polymerization of the functional monomer (methacrylic acid (MAA)), in the presence of a dummy template (Pro-Tyr-Ile-Leu (PYIL)), a crosslinker and porogens. The resulting monoliths were characterized by scanning electron microscopy, pore size distribution measurement, and Fourier transform infrared spectroscopy. Different synthesis conditions of the MIM column were investigated. The parameters affecting the MIM-CME performance, including loading, washing and elution protocols, were optimized as well. The MIMs were used to enrich NT and NmN from human plasma prior to HPLC-UV analysis. The imprinted monolith showed an excellent maximum adsorption capacity of 245–711 mg mL−1 and selectivity (imprinting factor of 5.7–13.4) towards its target peptides. Low detection limits of 0.62 and 1.20 nM, and satisfactory recoveries (82.5–98.8%) were obtained for NT and NmN, respectively. The proposed MIM-CME/HPLC-UV method was found suitable to be used as an effective tool for the highly efficient and specific analysis of NT and NmN in human plasma samples.

KW - Neuropeptides

KW - Molecularly imprinted monolithic column

KW - Capillary microextraction

KW - Epitope approach

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DO - 10.1016/j.talanta.2022.123397

M3 - Article

SN - 0039-9140

VL - 243

JO - Talanta

JF - Talanta

M1 - 123397

ER -

Fabrication of a molecularly imprinted monolithic column via the epitope approach for the selective capillary microextraction of neuropeptides in human plasma

Abstract

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Keywords

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