Fast and Accurate Identification of Dermatophytes by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Validation in the Clinical Laboratory

Ann Packeu; Annelies De Bel; C. Weil-Olivier; S Ranque; M Detandt; M Hendrickx

doi:10.1128/JCM.01428-14

Fast and Accurate Identification of Dermatophytes by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Validation in the Clinical Laboratory

Ann Packeu, Annelies De Bel, C. Weil-Olivier, S Ranque, M Detandt, M Hendrickx

Immunology and Microbiology

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

The performance of a matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) workflow using an extensive reference database for dermatophyte identification was evaluated on 176 clinical strains. Using a direct-deposit procedure after 3 incubation days yielded 40% correct identification. Both increasing incubation time and using an extraction procedure resulted in 100% correct identification.

Original language	English
Pages (from-to)	3440-3443
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	52
Issue number	9
DOIs	https://doi.org/10.1128/JCM.01428-14
Publication status	Published - Sep 2014

Keywords

Maldi-tof

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10.1128/JCM.01428-14

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title = "Fast and Accurate Identification of Dermatophytes by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Validation in the Clinical Laboratory",

abstract = "The performance of a matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) workflow using an extensive reference database for dermatophyte identification was evaluated on 176 clinical strains. Using a direct-deposit procedure after 3 incubation days yielded 40% correct identification. Both increasing incubation time and using an extraction procedure resulted in 100% correct identification.",

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author = "Ann Packeu and {De Bel}, Annelies and C. Weil-Olivier and S Ranque and M Detandt and M Hendrickx",

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pages = "3440--3443",

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Fast and Accurate Identification of Dermatophytes by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Validation in the Clinical Laboratory. / Packeu, Ann; De Bel, Annelies; Weil-Olivier, C.; Ranque, S; Detandt, M; Hendrickx, M.

In: Journal of Clinical Microbiology, Vol. 52, No. 9, 09.2014, p. 3440-3443.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Fast and Accurate Identification of Dermatophytes by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Validation in the Clinical Laboratory

AU - Packeu, Ann

AU - De Bel, Annelies

AU - Weil-Olivier, C.

AU - Ranque, S

AU - Detandt, M

AU - Hendrickx, M

PY - 2014/9

Y1 - 2014/9

N2 - The performance of a matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) workflow using an extensive reference database for dermatophyte identification was evaluated on 176 clinical strains. Using a direct-deposit procedure after 3 incubation days yielded 40% correct identification. Both increasing incubation time and using an extraction procedure resulted in 100% correct identification.

AB - The performance of a matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) workflow using an extensive reference database for dermatophyte identification was evaluated on 176 clinical strains. Using a direct-deposit procedure after 3 incubation days yielded 40% correct identification. Both increasing incubation time and using an extraction procedure resulted in 100% correct identification.

KW - Maldi-tof

U2 - 10.1128/JCM.01428-14

DO - 10.1128/JCM.01428-14

M3 - Article

VL - 52

SP - 3440

EP - 3443

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 9

ER -

Fast and Accurate Identification of Dermatophytes by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Validation in the Clinical Laboratory

Abstract

Keywords

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