Functionalization of gold nanoparticles with nanobodies through physical adsorption

Julie Goossens; Hla Sein; Shaohong Lu; Magdalena Radwanska; Serge Muyldermans; Yann G.J. Sterckx; Stefan Magez

doi:10.1039/c7ay00854f

Functionalization of gold nanoparticles with nanobodies through physical adsorption

Julie Goossens, Hla Sein, Shaohong Lu, Magdalena Radwanska, Serge Muyldermans, Yann G.J. Sterckx, Stefan Magez

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

Nanobodies (Nbs) are single-domain antibodies with highly desirable properties employed as versatile tools in structural biology, biochemistry and biomedical sciences. Nbs also have a significant potential for the development of highly specific and user-friendly diagnostic assays. These immuno-assays often employ gold nanoparticles (AuNPs) as a detection label. Despite great advances in Nb technology and the immuno-sensor field, the functionalization of AuNPs with Nbs mostly remains unchartered territory. In this paper, we present a study of the functionalization of AuNPs with Nbs through physical adsorption. We show that Nb pI, solution pH, additives and protein engineering strategies have profound effects on the generation of stable Nb-AuNPs. Our results further demonstrate that physical adsorption can be used to produce functional Nb-AuNPs that could be employed in a wide array of applications.

Original language	English
Pages (from-to)	3430-3440
Number of pages	11
Journal	Analytical Methods
Volume	9
Issue number	23
DOIs	https://doi.org/10.1039/c7ay00854f
Publication status	Published - 21 Jun 2017

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AU - Sein, Hla

AU - Lu, Shaohong

AU - Radwanska, Magdalena

AU - Muyldermans, Serge

AU - Sterckx, Yann G.J.

AU - Magez, Stefan

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Functionalization of gold nanoparticles with nanobodies through physical adsorption

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