Functionalized Cellulose Nanocrystals for Cellular Labeling and Bioimaging

Sebastian Raja; Ahmed E.I. Hamouda; Marcelo A.S. De Toledo; Chaolei Hu; Marcela P. Bernardo; Carmen Schalla; Liliane S.F. Leite; Eva Miriam Buhl; Stephan Dreschers; Andrij Pich; Martin Zenke; Luiz H.C. Mattoso; Antonio Sechi

doi:10.1021/acs.biomac.0c01317

Functionalized Cellulose Nanocrystals for Cellular Labeling and Bioimaging

Sebastian Raja, Ahmed E.I. Hamouda, Marcelo A.S. De Toledo, Chaolei Hu, Marcela P. Bernardo, Carmen Schalla, Liliane S.F. Leite, Eva Miriam Buhl, Stephan Dreschers, Andrij Pich, Martin Zenke, Luiz H.C. Mattoso, Antonio Sechi

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Cellulose nanocrystals (CNCs) are unique and promising natural nanomaterials that can be extracted from native cellulose fibers by acid hydrolysis. In this study, we developed chemically modified CNC derivatives by covalent tethering of PEGylated biotin and perylenediimide (PDI)-based near-infrared organic dye and evaluated their suitability for labeling and imaging of different cell lines including J774A.1 macrophages, NIH-3T3 fibroblasts, HeLa adenocarcinoma cells, and primary murine dendritic cells. PDI-labeled CNCs showed a superior photostability compared to similar commercially available dyes under long periods of constant and high-intensity illumination. All CNC derivatives displayed excellent cytocompatibility toward all cell types and efficiently labeled cells in a dose-dependent manner. Moreover, CNCs were effectively internalized and localized in the cytoplasm around perinuclear areas. Thus, our findings demonstrate the suitability of these new CNC derivatives for labeling, imaging, and long-time tracking of a variety of cell lines and primary cells.

Original language	English
Pages (from-to)	454-466
Number of pages	13
Journal	Biomacromolecules
Volume	22
Issue number	2
DOIs	https://doi.org/10.1021/acs.biomac.0c01317
Publication status	Published - 8 Feb 2021

Bibliographical note

Funding Information:
The authors thank FAPESP (proc. no. 2015/00094-0; proc. no. 2017/22017-3; proc. no. 2016/03080-3), CNPq (303.796/2014-6; 402.287/2013-4), MCTI/SISNANO, REDEAGRONANO, and CAPES-Alexander von Humboldt postdoctoral fellowship (no. 99999.001703/2014-05 to M.A.S.T.) for financial support. The authors also thank S. Gründer (Institute of Physiology, RWTH Aachen University) and his lab members for providing the opportunity to use their confocal microscope.

Publisher Copyright:
© 2020 American Chemical Society.

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title = "Functionalized Cellulose Nanocrystals for Cellular Labeling and Bioimaging",

abstract = "Cellulose nanocrystals (CNCs) are unique and promising natural nanomaterials that can be extracted from native cellulose fibers by acid hydrolysis. In this study, we developed chemically modified CNC derivatives by covalent tethering of PEGylated biotin and perylenediimide (PDI)-based near-infrared organic dye and evaluated their suitability for labeling and imaging of different cell lines including J774A.1 macrophages, NIH-3T3 fibroblasts, HeLa adenocarcinoma cells, and primary murine dendritic cells. PDI-labeled CNCs showed a superior photostability compared to similar commercially available dyes under long periods of constant and high-intensity illumination. All CNC derivatives displayed excellent cytocompatibility toward all cell types and efficiently labeled cells in a dose-dependent manner. Moreover, CNCs were effectively internalized and localized in the cytoplasm around perinuclear areas. Thus, our findings demonstrate the suitability of these new CNC derivatives for labeling, imaging, and long-time tracking of a variety of cell lines and primary cells.",

author = "Sebastian Raja and Hamouda, {Ahmed E.I.} and {De Toledo}, {Marcelo A.S.} and Chaolei Hu and Bernardo, {Marcela P.} and Carmen Schalla and Leite, {Liliane S.F.} and Buhl, {Eva Miriam} and Stephan Dreschers and Andrij Pich and Martin Zenke and Mattoso, {Luiz H.C.} and Antonio Sechi",

note = "Funding Information: The authors thank FAPESP (proc. no. 2015/00094-0; proc. no. 2017/22017-3; proc. no. 2016/03080-3), CNPq (303.796/2014-6; 402.287/2013-4), MCTI/SISNANO, REDEAGRONANO, and CAPES-Alexander von Humboldt postdoctoral fellowship (no. 99999.001703/2014-05 to M.A.S.T.) for financial support. The authors also thank S. Gr{\"u}nder (Institute of Physiology, RWTH Aachen University) and his lab members for providing the opportunity to use their confocal microscope. Publisher Copyright: {\textcopyright} 2020 American Chemical Society.",

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doi = "10.1021/acs.biomac.0c01317",

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AU - Raja, Sebastian

AU - Hamouda, Ahmed E.I.

AU - De Toledo, Marcelo A.S.

AU - Hu, Chaolei

AU - Bernardo, Marcela P.

AU - Schalla, Carmen

AU - Leite, Liliane S.F.

AU - Buhl, Eva Miriam

AU - Dreschers, Stephan

AU - Pich, Andrij

AU - Zenke, Martin

AU - Mattoso, Luiz H.C.

AU - Sechi, Antonio

N1 - Funding Information: The authors thank FAPESP (proc. no. 2015/00094-0; proc. no. 2017/22017-3; proc. no. 2016/03080-3), CNPq (303.796/2014-6; 402.287/2013-4), MCTI/SISNANO, REDEAGRONANO, and CAPES-Alexander von Humboldt postdoctoral fellowship (no. 99999.001703/2014-05 to M.A.S.T.) for financial support. The authors also thank S. Gründer (Institute of Physiology, RWTH Aachen University) and his lab members for providing the opportunity to use their confocal microscope. Publisher Copyright: © 2020 American Chemical Society.

PY - 2021/2/8

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N2 - Cellulose nanocrystals (CNCs) are unique and promising natural nanomaterials that can be extracted from native cellulose fibers by acid hydrolysis. In this study, we developed chemically modified CNC derivatives by covalent tethering of PEGylated biotin and perylenediimide (PDI)-based near-infrared organic dye and evaluated their suitability for labeling and imaging of different cell lines including J774A.1 macrophages, NIH-3T3 fibroblasts, HeLa adenocarcinoma cells, and primary murine dendritic cells. PDI-labeled CNCs showed a superior photostability compared to similar commercially available dyes under long periods of constant and high-intensity illumination. All CNC derivatives displayed excellent cytocompatibility toward all cell types and efficiently labeled cells in a dose-dependent manner. Moreover, CNCs were effectively internalized and localized in the cytoplasm around perinuclear areas. Thus, our findings demonstrate the suitability of these new CNC derivatives for labeling, imaging, and long-time tracking of a variety of cell lines and primary cells.

AB - Cellulose nanocrystals (CNCs) are unique and promising natural nanomaterials that can be extracted from native cellulose fibers by acid hydrolysis. In this study, we developed chemically modified CNC derivatives by covalent tethering of PEGylated biotin and perylenediimide (PDI)-based near-infrared organic dye and evaluated their suitability for labeling and imaging of different cell lines including J774A.1 macrophages, NIH-3T3 fibroblasts, HeLa adenocarcinoma cells, and primary murine dendritic cells. PDI-labeled CNCs showed a superior photostability compared to similar commercially available dyes under long periods of constant and high-intensity illumination. All CNC derivatives displayed excellent cytocompatibility toward all cell types and efficiently labeled cells in a dose-dependent manner. Moreover, CNCs were effectively internalized and localized in the cytoplasm around perinuclear areas. Thus, our findings demonstrate the suitability of these new CNC derivatives for labeling, imaging, and long-time tracking of a variety of cell lines and primary cells.

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ER -

Functionalized Cellulose Nanocrystals for Cellular Labeling and Bioimaging

Abstract

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