Two subtypes of Theiler's murine encephalomyelitis virus (TMEV), belonging to the Picornaviridae, induce a particular pathology in the central nervous system of mice. Based on this difference, we distinguish the neurovirulent strains (e.g. GDVII) that cause an acute and mostly fatal encephalomyelitis, whereas the demeylinating subtypes (e.g. DA) develop chronic and progressive symptoms that resemble the human disease multiple sclerosis. Our lab succeeded to cultivate a house-made persistent cell line, called DRAW, after infection of RAW macrophages with the DA-strain. Viral persistence is considered to be an important disease determinant that leads to chronic inflammation, demyelination and auto-immunity. That makes our in vitro model appropriate to study the molecular mechanism of viral persistence and to clarify the cellular processes that are involved. Total RNA was extracted from RAW and DRAW cells at time 0h (control) and 48h (maximal virus replication). A microarray study revealed ten to hundreds of differentially expressed probes, depending on the conditions that were compared. These differences will be interpreted into cellular relevant processes.
|Title of host publication||Unknown|
|Publication status||Published - 2010|
- gene expression