Genetic characterization of Shigatoxigenic and enteropathogenic Escherichia coli O80:H2 from diarrhoeic and septicaemic calves and relatedness to human Shigatoxigenic E. coli O80:H2

A Habets, F Crombé, K Nakamura, V Guérin, K De Rauw, D Piérard, M Saulmont, T Hayashi, J G Mainil, D Thiry

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Abstract

AIM: The purpose of this work was to identify and genetically characterize enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) O80:H2 from diarrhoeic and septicaemic calves in Belgium and to comparing them with human EHEC after whole genome sequencing.

METHODS AND RESULTS: Ten EHEC and 21 EPEC O80 identified by PCR between 2009 and 2018 from faeces, intestinal content and a kidney of diarrhoeic or septicaemic calves were genome sequenced and compared to 19 human EHEC identified between 2008 and 2019. They all belonged to the O80:H2 serotype and ST301, harboured the eaeξ gene, and 23 of the 29 EHEC contained the stx2d gene. Phylogenetically, they were distributed in two major sub-lineages: one comprised a majority of bovine EPEC whereas the second one comprised a majority of stx2d bovine and human EHEC.

CONCLUSIONS: Not only EPEC but also EHEC O80:H2 are present in diarrhoeic and septicaemic calves in Belgium and are genetically related to human EHEC.

SIGNIFICANCE AND IMPACT OF THE STUDY: These findings support the need to assess cattle as potential source of contamination of humans by EHEC O80:H2 and to understand the evolution of bovine and human EHEC and EPEC O80:H2.

Original languageEnglish
Pages (from-to)258-264
Number of pages7
JournalJournal of Applied Microbiology
Volume130
Issue number1
DOIs
Publication statusPublished - Jan 2021

Bibliographical note

© 2020 The Society for Applied Microbiology.

Keywords

  • Animals
  • Belgium/epidemiology
  • Cattle
  • Cattle Diseases/epidemiology
  • Diarrhea/epidemiology
  • Enterohemorrhagic Escherichia coli/classification
  • Enteropathogenic Escherichia coli/classification
  • Escherichia coli Infections/epidemiology
  • Escherichia coli Proteins/genetics
  • Genome, Bacterial/genetics
  • Humans
  • Phylogeny
  • Sepsis/epidemiology
  • Serogroup

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