Genetic variation in three candidate genes and nicotine dependence, withdrawal and smoking cessation in hospitalized patients

Kim De Ruyck, Kristiaan Nackaerts, Laurence Beels, Joke Werbrouck, Annick De Volder, Marc Meysman, Bihiyga Salhi, Jan Van Meerbeeck, Hubert Thierens

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)


AIMS: This study evaluates the relationship of six polymorphisms found in the CHRNA3, DRD2 and COMT genes with nicotine dependence, the ability to quit smoking and the occurrence of withdrawal symptoms after short-term use of nicotine patch in hospitalized patients.

MATERIALS & METHODS: The study included 233 participants from a double-blind, placebo-controlled trial of nicotine patch substitution with a 6-month follow-up period. Nicotine dependence was assessed by the Fagerström Test for Nicotine Dependence (FTND) questionnaire, withdrawal symptoms by the Minnesota Nicotine Withdrawal Scale questionnaire and smoking cessation by self-reported abstinence at 1 week, 1 month and 6 months after treatment.

RESULTS: After correcting for multiple testing, three polymorphisms in the DRD2 gene (Taq1A, Taq1B and Pro319Pro) were significantly associated with nicotine dependence (p = 0.018, p = 0.048 and p = 0.006, respectively). Using a cutoff point for the FTND score, the CHRNA3 Tyr215Tyr (rs1051730) polymorphism was also associated with nicotine dependence (p = 0.037 and p = 0.074 after correction for multiple testing). No association of any of the studied polymorphisms was observed with either smoking cessation or the occurrence of withdrawal symptoms.

CONCLUSION: This study confirms the reported association of the CHRNA3 locus with nicotine dependence and shows the involvement of two independent DRD2 polymorphisms in nicotine dependence.

Original languageEnglish
Pages (from-to)1053-1063
Number of pages11
Issue number8
Publication statusPublished - Aug 2010


  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Catechol O-Methyltransferase/genetics
  • Double-Blind Method
  • Female
  • Hospitalization
  • Humans
  • Male
  • Middle Aged
  • Nicotine/administration & dosage
  • Polymorphism, Genetic
  • Receptors, Dopamine D2/genetics
  • Receptors, Nicotinic/genetics
  • Smoking Cessation/methods
  • Substance Withdrawal Syndrome/genetics
  • Surveys and Questionnaires
  • Tobacco Use Disorder/genetics
  • Transdermal Patch
  • Treatment Outcome
  • Young Adult


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