Immunohisto- and cytochemistry analysis of connexins

Bruno Cogliati; Michaël Maes; Isabel Veloso Alves Pereira; Joost Willebrords; Tereza Cristina da Silva; Sara Crespo Yanguas; Mathieu Vinken

Immunohisto- and cytochemistry analysis of connexins

Bruno Cogliati, Michaël Maes, Isabel Veloso Alves Pereira, Joost Willebrords, Tereza Cristina da Silva, Sara Crespo Yanguas, Mathieu Vinken

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

4 Citations (Scopus)

Abstract

Immunohistochemistry (IHC) is a ubiquitous used technique to identify and analyze protein expression in the context of tissue and cell morphology. In the connexin research field, IHC is applied to identify the subcellular location of connexin proteins, as this can be directly linked to their functionality. The present chapter describes a protocol for fluorescent IHC to detect connexin proteins in tissues slices and cells, with slight modifications depending on the nature of biological sample, histological processing and/or protein expression level. Basically, fluorescent IHC is a short, simple and cost-effective technique, which allows the visualization of proteins based on fluorescent-labeled antibody-antigen recognition.

Original language	English
Title of host publication	Gap Junction Protocols
Editors	Mathieu Vinken, Scott Johnstone
Place of Publication	New York
Publisher	Springer Protocols
Pages	55-70
ISBN (Print)	978-1-4939-3664-9
Publication status	Published - 22 May 2016

Keywords

connexin
antibody
immunohistochemistry
immunocytochemistry
microscopy
morphology
fluorophores
localization

OZR2651: International Joint Research Group - Liver Connexin and Pannexin Research Group (LCPR)
Vinken, M., Rogiers, V., Cogliati, B. & Dagli, M. L.
1/08/14 → 1/07/26
Project: Fundamental

The role of connexins, pannexins and their channels in liver fibrosis
Sara Crespo Yanguas (Speaker)
28 Nov 2018
Activity: Talk or presentation › Talk or presentation at a workshop/seminar

Cite this

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title = "Immunohisto- and cytochemistry analysis of connexins",

abstract = "Immunohistochemistry (IHC) is a ubiquitous used technique to identify and analyze protein expression in the context of tissue and cell morphology. In the connexin research field, IHC is applied to identify the subcellular location of connexin proteins, as this can be directly linked to their functionality. The present chapter describes a protocol for fluorescent IHC to detect connexin proteins in tissues slices and cells, with slight modifications depending on the nature of biological sample, histological processing and/or protein expression level. Basically, fluorescent IHC is a short, simple and cost-effective technique, which allows the visualization of proteins based on fluorescent-labeled antibody-antigen recognition. ",

keywords = "connexin, antibody, immunohistochemistry, immunocytochemistry, microscopy, morphology, fluorophores, localization",

author = "Bruno Cogliati and Micha{\"e}l Maes and {Alves Pereira}, {Isabel Veloso} and Joost Willebrords and {da Silva}, {Tereza Cristina} and {Crespo Yanguas}, Sara and Mathieu Vinken",

year = "2016",

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day = "22",

language = "English",

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Immunohisto- and cytochemistry analysis of connexins. / Cogliati, Bruno; Maes, Michaël; Alves Pereira, Isabel Veloso; Willebrords, Joost; da Silva, Tereza Cristina; Crespo Yanguas, Sara; Vinken, Mathieu.

Gap Junction Protocols. ed. / Mathieu Vinken; Scott Johnstone. New York : Springer Protocols, 2016. p. 55-70.

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - Immunohisto- and cytochemistry analysis of connexins

AU - Cogliati, Bruno

AU - Maes, Michaël

AU - Alves Pereira, Isabel Veloso

AU - Willebrords, Joost

AU - da Silva, Tereza Cristina

AU - Crespo Yanguas, Sara

AU - Vinken, Mathieu

PY - 2016/5/22

Y1 - 2016/5/22

N2 - Immunohistochemistry (IHC) is a ubiquitous used technique to identify and analyze protein expression in the context of tissue and cell morphology. In the connexin research field, IHC is applied to identify the subcellular location of connexin proteins, as this can be directly linked to their functionality. The present chapter describes a protocol for fluorescent IHC to detect connexin proteins in tissues slices and cells, with slight modifications depending on the nature of biological sample, histological processing and/or protein expression level. Basically, fluorescent IHC is a short, simple and cost-effective technique, which allows the visualization of proteins based on fluorescent-labeled antibody-antigen recognition.

AB - Immunohistochemistry (IHC) is a ubiquitous used technique to identify and analyze protein expression in the context of tissue and cell morphology. In the connexin research field, IHC is applied to identify the subcellular location of connexin proteins, as this can be directly linked to their functionality. The present chapter describes a protocol for fluorescent IHC to detect connexin proteins in tissues slices and cells, with slight modifications depending on the nature of biological sample, histological processing and/or protein expression level. Basically, fluorescent IHC is a short, simple and cost-effective technique, which allows the visualization of proteins based on fluorescent-labeled antibody-antigen recognition.

KW - connexin

KW - antibody

KW - immunohistochemistry

KW - immunocytochemistry

KW - microscopy

KW - morphology

KW - fluorophores

KW - localization

M3 - Chapter

SN - 978-1-4939-3664-9

SP - 55

EP - 70

BT - Gap Junction Protocols

A2 - Vinken, Mathieu

A2 - Johnstone, Scott

PB - Springer Protocols

CY - New York

ER -

Immunohisto- and cytochemistry analysis of connexins

Abstract

Keywords

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Projects

OZR2651: International Joint Research Group - Liver Connexin and Pannexin Research Group (LCPR)

Activities

The role of connexins, pannexins and their channels in liver fibrosis

Cite this