Impact of maternal pertussis antibodies on the infants' cellular immune responses

Marjolein R P Orije, Irene García-Fogeda, Wouter Van Dyck, Véronique Corbière, Françoise Mascart, Ludo Mahieu, Niel Hens, Pierre Van Damme, Nathalie Cools, Benson Ogunjimi, Kirsten Maertens, Elke Leuridan

Research output: Contribution to journalArticlepeer-review

Abstract

INTRODUCTION: Maternal antibody interference of the infant's humoral immune responses raises some concern to the strategy of maternal Tdap (tetanus, diphtheria, acellular pertussis [aP]) vaccination. This study assessed the impact of maternal Tdap antibodies on the infant's pertussis-specific T lymphocyte responses following infant vaccination with an aP containing vaccine, in a term and preterm born cohort.

METHODS: Heparin samples (±0.5mL) were conveniently drawn from infants of a Belgian prospective cohort study (N=79, NCT02511327), including Tdap vaccinated (Boostrix®) and non-vaccinated women (no Tdap vaccine in the last 5 years) that delivered at term or prematurely. Sampling was performed before and one month after primary (8-12-16 weeks) and booster vaccination (13 or 15 months) with DTaP-IPV-HB-PRP~T vaccine (Hexyon®). Pertussis toxin (PT)-specific CD3 +, CD3 +CD4 + and CD3 +CD8 + lymphoblasts and their cytokine secretions were measured using a flow cytometric assay on whole blood (FASCIA) and multiplex technology (Meso Scale Discovery), respectively.

RESULTS: 57% of all infants were considered PT-specific CD3 +CD4 + lymphoblasts responders after primary and booster vaccination, whereas 17% were CD3 +CD8 + lymphoblast responders. IFN-γ, IL-13, IL-17A and IL-5 cytokine secretions after primary and booster vaccination were indicative of a mixed T helper (Th) 1/Th2/Th17 cell profile. Lymphoblast and cytokine levels were comparable between term and preterm infants. Non-responders for IL-13 after booster vaccination had higher maternal PT IgG levels at birth when compared to responders.

CONCLUSIONS: Term and preterm born infants are capable of inducing Th1, Th2 and Th17 responses after aP vaccination, yet maternal vaccination modulate these responses. Evaluation of this effect in larger trials is needed.

Original languageEnglish
Pages (from-to)442-452
Number of pages11
JournalClinical Infectious Diseases
Volume75
Issue number3
Early online date24 Nov 2021
DOIs
Publication statusPublished - 31 Aug 2022

Bibliographical note

© The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

Keywords

  • cell-mediated immune response
  • maternal antibodies
  • maternal immunization
  • preterm born infants
  • Tdap

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