Impact of the sample matrix composition on the signal enhancement in the capillary electrophoretic separation of poliovirus samples

Iuliana Oita, Hadewych Halewyck, Bert Thys, Bartholomeus Rombaut, Yvan Vander Heyden

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The development of capillary electrophoretic applications aiming to provide reliable stability assessment
of viral suspensions, to detect subviral particles from cell extracts or to study the interactions
between virus particles and various biomolecules, cannot be done without a thorough understanding of
the sample matrix contribution to the observed electrophoretic behaviour. The present study thoroughly
investigates the effect of the sample matrix on the electrophoretic behaviour of poliovirus injected as
sample plugs of 1%, 5% and 12% effective capillary length. The effect of the sample matrix for three different
poliovirus batches was evaluated. Additionally, simulated samples, obtained from concentrated
poliovirus suspensions of high purity and diluted with commonly used lab buffers in order to obtain samples
with either high or low conductivities, were also investigated. The goal of the study was to obtain
a better understanding of the effect of the sample matrix on the signal enhancement, in order to define
a general approach allowing a repeatable capillary electrophoretic (CE) separation of poliovirus from
complex samples. This study clearly demonstrates that the sample matrix has an important influence on
the sensitivity of the CE poliovirus separations. Translation of these observations into routine practice
involves several compromises and a set of rules in order to reduce day-to-day variation and to maximize
sensitivity.
Original languageEnglish
Pages (from-to)42-50
Number of pages9
JournalAnalytica Chimica Acta
Volume747
Publication statusPublished - 2012

Keywords

  • sample matrix
  • capillary electrophoretic separation

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