In vitro multipotency of human bone marrow (mesenchymal) stem cells

Sarah Snykers; Tamara Vanhaecke; Peggy Papeleu; Tom Henkens; Mathieu Vinken; Greetje Elaut; Ivan Van Riet; Vera Rogiers

In vitro multipotency of human bone marrow (mesenchymal) stem cells

Sarah Snykers, Tamara Vanhaecke, Peggy Papeleu, Tom Henkens, Mathieu Vinken, Greetje Elaut, Ivan Van Riet, Vera Rogiers

Research output: Contribution to journal › Meeting abstract (Journal)

Abstract

The capability of mesenchymal stem cells (MSC) to differentiate in vitro into hepatocytes by exposure to liver-specific cytokines was investigated. Simultaneous exposure of MSC to a mixture of hepatogenic cytokines only stimulated neuroectodermal and mesodermal differentiation. However, sequential exposure, resembling the order of secretion during liver embryogenesis, induced both glycogen storage and expression of cytokeratin 18. In order to trigger further endodermal differentiation, cells were exposed to trichostatin A (TSA). The latter up-regulated albumin secretion, a typical functional property of primary hepatocytes. In conclusion, MSC acquire trilineage potential under ''hepatocyte-specific conditions''. TSA improves differentiation of MSC towards hepatocyte-like cells.

Original language	English
Pages (from-to)	400-405
Number of pages	6
Journal	ALTEX
Volume	22
Issue number	s1/06
Publication status	Published - 1 May 2006

Bibliographical note

Altex - Alternativen zur Tierexperimenten, vol. 23 (Special Issue), pag. 400-405

Keywords

bone marrow stem cells
hepatocytes
embryonic development
histone deacetylase inhibitor
in vitro

Cite this

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title = "In vitro multipotency of human bone marrow (mesenchymal) stem cells",

abstract = "The capability of mesenchymal stem cells (MSC) to differentiate in vitro into hepatocytes by exposure to liver-specific cytokines was investigated. Simultaneous exposure of MSC to a mixture of hepatogenic cytokines only stimulated neuroectodermal and mesodermal differentiation. However, sequential exposure, resembling the order of secretion during liver embryogenesis, induced both glycogen storage and expression of cytokeratin 18. In order to trigger further endodermal differentiation, cells were exposed to trichostatin A (TSA). The latter up-regulated albumin secretion, a typical functional property of primary hepatocytes. In conclusion, MSC acquire trilineage potential under ''hepatocyte-specific conditions''. TSA improves differentiation of MSC towards hepatocyte-like cells.",

keywords = "bone marrow stem cells, hepatocytes, embryonic development, histone deacetylase inhibitor, in vitro",

author = "Sarah Snykers and Tamara Vanhaecke and Peggy Papeleu and Tom Henkens and Mathieu Vinken and Greetje Elaut and {Van Riet}, Ivan and Vera Rogiers",

note = "Altex - Alternativen zur Tierexperimenten, vol. 23 (Special Issue), pag. 400-405",

year = "2006",

month = may,

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TY - JOUR

T1 - In vitro multipotency of human bone marrow (mesenchymal) stem cells

AU - Snykers, Sarah

AU - Vanhaecke, Tamara

AU - Papeleu, Peggy

AU - Henkens, Tom

AU - Vinken, Mathieu

AU - Elaut, Greetje

AU - Van Riet, Ivan

AU - Rogiers, Vera

N1 - Altex - Alternativen zur Tierexperimenten, vol. 23 (Special Issue), pag. 400-405

PY - 2006/5/1

Y1 - 2006/5/1

N2 - The capability of mesenchymal stem cells (MSC) to differentiate in vitro into hepatocytes by exposure to liver-specific cytokines was investigated. Simultaneous exposure of MSC to a mixture of hepatogenic cytokines only stimulated neuroectodermal and mesodermal differentiation. However, sequential exposure, resembling the order of secretion during liver embryogenesis, induced both glycogen storage and expression of cytokeratin 18. In order to trigger further endodermal differentiation, cells were exposed to trichostatin A (TSA). The latter up-regulated albumin secretion, a typical functional property of primary hepatocytes. In conclusion, MSC acquire trilineage potential under ''hepatocyte-specific conditions''. TSA improves differentiation of MSC towards hepatocyte-like cells.

AB - The capability of mesenchymal stem cells (MSC) to differentiate in vitro into hepatocytes by exposure to liver-specific cytokines was investigated. Simultaneous exposure of MSC to a mixture of hepatogenic cytokines only stimulated neuroectodermal and mesodermal differentiation. However, sequential exposure, resembling the order of secretion during liver embryogenesis, induced both glycogen storage and expression of cytokeratin 18. In order to trigger further endodermal differentiation, cells were exposed to trichostatin A (TSA). The latter up-regulated albumin secretion, a typical functional property of primary hepatocytes. In conclusion, MSC acquire trilineage potential under ''hepatocyte-specific conditions''. TSA improves differentiation of MSC towards hepatocyte-like cells.

KW - bone marrow stem cells

KW - hepatocytes

KW - embryonic development

KW - histone deacetylase inhibitor

KW - in vitro

M3 - Meeting abstract (Journal)

SN - 1868-596X

VL - 22

SP - 400

EP - 405

JO - ALTEX

JF - ALTEX

IS - s1/06

ER -

In vitro multipotency of human bone marrow (mesenchymal) stem cells

Abstract

Bibliographical note

Keywords

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