Influence of mild hypothermia on pro- and anti-inflammatory cytokines after a transient focal cerebral ischemia in rats

An-Gaelle Ceulemans, Tine Zgavc, Ron Kooijman, Said Hachimi-Idrissi, Sophie Sarre, Yvette Michotte

Research output: Chapter in Book/Report/Conference proceedingMeeting abstract (Book)

Abstract

Background: Hypothermia is a promising neuroprotective strategy to improve functional outcome after stroke. As inflammation plays an important role in the ischemic cascade, the effect of mild hypothermia on cytokines was investigated after a transient focal cerebral ischemia.

Material and Methods: Endothelin-1 (Et-1), a vasoconstrictor, was infused in the vicinity of the middle cerebral artery to elicit a transient focal cerebral ischemia in male Wistar rats. Rats subjected to two hours of mild hypothermia starting at 20 min after Et-1 injection, were compared to a normothermic group. The assessed parameters were functional outcome (neurological deficit score), infarct volume and the concentrations of inflammatory (Interleukin-1 beta, Tumor Necrosis Factor alpha) and anti-inflammatory (Transforming Growth Factor beta) cytokines at different time points after stroke.

Results: Hypothermia significantly reduces the infarct volume compared to normothermia. This reduction correlates with behaviour as hypothermic rats show a better outcome after the insult. However, even in normothermic rats, neurological deficit improves in time. Stroke induces an increase in pro-inflammatory cytokines in the core and the penumbra that peaks at 24 hours after the insult. Application of hypothermia leads to increased levels of Tumor Necrosis Factor alpha at 8 hours after the insult and decreased levels of the pro-inflammatory cytokines at 24 hours. In contrast, hypothermia had no effect on the stroke induced increase in Transforming Growth Factor beta.

Conclusion: These results suggest that beneficial effects of hypothermia on infarct volume and functional outcome may be, at least in part, mediated by modulation of inflammatory cytokine expression.
Original languageEnglish
Title of host publicationProgram book of the "3rd International Hypothermia Symposium"
EditorsHans Friberg, Tadeusz Wieloch
Pages24-24
Number of pages1
Publication statusPublished - 2 Sep 2009
EventFinds and Results from the Swedish Cyprus Expedition: A Gender Perspective at the Medelhavsmuseet - Stockholm, Sweden
Duration: 21 Sep 200925 Sep 2009

Publication series

NameProgram book of the "3rd International Hypothermia Symposium"

Conference

ConferenceFinds and Results from the Swedish Cyprus Expedition: A Gender Perspective at the Medelhavsmuseet
Country/TerritorySweden
CityStockholm
Period21/09/0925/09/09

Bibliographical note

Hans Friberg and Tadeusz Wieloch

Keywords

  • hypothermia
  • stroke
  • inflammation

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