TY - JOUR
T1 - Involvement of the complement system in the pathogenesis of pulmonary leukostasis in experimental myelocytic leukemia
AU - Van Buchem, Mark A.
AU - Levelt, Christiaan N.
AU - Hogendoorn, Paneras C.W.
AU - Colly, Louisa P.
AU - Kluin, Philip M.
AU - Willemze, Roel
AU - Daha, Mohamed R.
PY - 1993/10
Y1 - 1993/10
N2 - The role of the complement system in the pathogenesis of pulmonary leukostasis in myelocytic leukemia was studied in a rat model. Acute myelocytic leukemia was induced in six Brown-Norway rats, and complement levels were assayed during the course of the disease. Whole complement activity (CH50) and hemolytic activity of C1q, C3, and C4 decreased from day 16 after induction of the leukemia, when the rats developed pulmonary leukostasis. In addition, local complement activation was established in the lung vessels by immunofluorescence microscopy in advanced stages of pulmonary leukostasis. Finally, following systemic activation of the complement system by injection of cobra venom factor (CVF), leukemic rats (n = 6) died of pulmonary leukostasis 4.5 days earlier than did leukemic controls (n = 6). These findings suggest that, in acute myelocytic leukemia in Brown-Norway rats, pulmonary leukostasis is induced by activation of the complement system. This finding could lead to new modes of treatment for a life-threatening complication of leukemia.
AB - The role of the complement system in the pathogenesis of pulmonary leukostasis in myelocytic leukemia was studied in a rat model. Acute myelocytic leukemia was induced in six Brown-Norway rats, and complement levels were assayed during the course of the disease. Whole complement activity (CH50) and hemolytic activity of C1q, C3, and C4 decreased from day 16 after induction of the leukemia, when the rats developed pulmonary leukostasis. In addition, local complement activation was established in the lung vessels by immunofluorescence microscopy in advanced stages of pulmonary leukostasis. Finally, following systemic activation of the complement system by injection of cobra venom factor (CVF), leukemic rats (n = 6) died of pulmonary leukostasis 4.5 days earlier than did leukemic controls (n = 6). These findings suggest that, in acute myelocytic leukemia in Brown-Norway rats, pulmonary leukostasis is induced by activation of the complement system. This finding could lead to new modes of treatment for a life-threatening complication of leukemia.
UR - http://www.scopus.com/inward/record.url?scp=0027372061&partnerID=8YFLogxK
M3 - Article
C2 - 8412322
AN - SCOPUS:0027372061
SN - 0887-6924
VL - 7
SP - 1608
EP - 1614
JO - Leukemia
JF - Leukemia
IS - 10
ER -