Liquid Biopsies as Non-Invasive Tools for Mutation Profiling in Multiple Myeloma: Application Potential, Challenges, and Opportunities

Robbe Heestermans; Rik Schots; Ann De Becker; Ivan Van Riet

doi:10.3390/ijms25105208

Liquid Biopsies as Non-Invasive Tools for Mutation Profiling in Multiple Myeloma: Application Potential, Challenges, and Opportunities

Robbe Heestermans, Rik Schots, Ann De Becker, Ivan Van Riet

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

17 Downloads (Pure)

Abstract

Over the last decades, the survival of multiple myeloma (MM) patients has considerably improved. However, despite the availability of new treatments, most patients still relapse and become therapy-resistant at some point in the disease evolution. The mutation profile has an impact on MM patients’ outcome, while typically evolving over time. Because of the patchy bone marrow (BM) infiltration pattern, the analysis of a single bone marrow sample can lead to an underestimation of the known genetic heterogeneity in MM. As a result, interest is shifting towards blood-derived liquid biopsies, which allow for a more comprehensive and non-invasive genetic interrogation without the discomfort of repeated BM aspirations. In this review, we compare the application potential for mutation profiling in MM of circulating-tumor-cell-derived DNA, cell-free DNA and extracellular-vesicle-derived DNA, while also addressing the challenges associated with their use. 

Original language	English
Article number	5208
Number of pages	15
Journal	International Journal of Molecular Sciences
Volume	25
Issue number	10
DOIs	https://doi.org/10.3390/ijms25105208
Publication status	Published - 10 May 2024

Bibliographical note

Funding Information:
Robbe Heestermans is employed as a Predoctoral Fellow by the Research Foundation Flanders (FWO) (1SE9324N). This research is supported by Kom op tegen Kanker, the Research Foundation Flanders (FWO), and the UZ Brussel Foundation.

Publisher Copyright:
© 2024 by the authors.

Keywords

Liquid biopsy
Multiple Myeloma
mutation profiling
Cell-free DNA
Circulating tumor cells
Extracellular vesicles
Personalized Medicine

Access to Document

10.3390/ijms25105208Licence: CC BY

111320072Final published version, 991 KBLicence: CC BY

Cite this

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title = "Liquid Biopsies as Non-Invasive Tools for Mutation Profiling in Multiple Myeloma: Application Potential, Challenges, and Opportunities",

abstract = "Over the last decades, the survival of multiple myeloma (MM) patients has considerably improved. However, despite the availability of new treatments, most patients still relapse and become therapy-resistant at some point in the disease evolution. The mutation profile has an impact on MM patients{\textquoteright} outcome, while typically evolving over time. Because of the patchy bone marrow (BM) infiltration pattern, the analysis of a single bone marrow sample can lead to an underestimation of the known genetic heterogeneity in MM. As a result, interest is shifting towards blood-derived liquid biopsies, which allow for a more comprehensive and non-invasive genetic interrogation without the discomfort of repeated BM aspirations. In this review, we compare the application potential for mutation profiling in MM of circulating-tumor-cell-derived DNA, cell-free DNA and extracellular-vesicle-derived DNA, while also addressing the challenges associated with their use. ",

keywords = "Liquid biopsy, Multiple Myeloma, mutation profiling, Cell-free DNA, Circulating tumor cells, Extracellular vesicles, Personalized Medicine",

author = "Robbe Heestermans and Rik Schots and {De Becker}, Ann and {Van Riet}, Ivan",

note = "Funding Information: Robbe Heestermans is employed as a Predoctoral Fellow by the Research Foundation Flanders (FWO) (1SE9324N). This research is supported by Kom op tegen Kanker, the Research Foundation Flanders (FWO), and the UZ Brussel Foundation. Publisher Copyright: {\textcopyright} 2024 by the authors.",

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volume = "25",

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AU - Schots, Rik

AU - De Becker, Ann

AU - Van Riet, Ivan

N1 - Funding Information: Robbe Heestermans is employed as a Predoctoral Fellow by the Research Foundation Flanders (FWO) (1SE9324N). This research is supported by Kom op tegen Kanker, the Research Foundation Flanders (FWO), and the UZ Brussel Foundation. Publisher Copyright: © 2024 by the authors.

PY - 2024/5/10

Y1 - 2024/5/10

N2 - Over the last decades, the survival of multiple myeloma (MM) patients has considerably improved. However, despite the availability of new treatments, most patients still relapse and become therapy-resistant at some point in the disease evolution. The mutation profile has an impact on MM patients’ outcome, while typically evolving over time. Because of the patchy bone marrow (BM) infiltration pattern, the analysis of a single bone marrow sample can lead to an underestimation of the known genetic heterogeneity in MM. As a result, interest is shifting towards blood-derived liquid biopsies, which allow for a more comprehensive and non-invasive genetic interrogation without the discomfort of repeated BM aspirations. In this review, we compare the application potential for mutation profiling in MM of circulating-tumor-cell-derived DNA, cell-free DNA and extracellular-vesicle-derived DNA, while also addressing the challenges associated with their use. 

AB - Over the last decades, the survival of multiple myeloma (MM) patients has considerably improved. However, despite the availability of new treatments, most patients still relapse and become therapy-resistant at some point in the disease evolution. The mutation profile has an impact on MM patients’ outcome, while typically evolving over time. Because of the patchy bone marrow (BM) infiltration pattern, the analysis of a single bone marrow sample can lead to an underestimation of the known genetic heterogeneity in MM. As a result, interest is shifting towards blood-derived liquid biopsies, which allow for a more comprehensive and non-invasive genetic interrogation without the discomfort of repeated BM aspirations. In this review, we compare the application potential for mutation profiling in MM of circulating-tumor-cell-derived DNA, cell-free DNA and extracellular-vesicle-derived DNA, while also addressing the challenges associated with their use. 

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KW - Multiple Myeloma

KW - mutation profiling

KW - Cell-free DNA

KW - Circulating tumor cells

KW - Extracellular vesicles

KW - Personalized Medicine

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DO - 10.3390/ijms25105208

M3 - Article

SN - 1422-0067

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JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

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M1 - 5208

ER -

Liquid Biopsies as Non-Invasive Tools for Mutation Profiling in Multiple Myeloma: Application Potential, Challenges, and Opportunities

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

FWOSB118: Tumor characterization and disease follow-up in multiple myeloma by (epi) genetic profiling using liquid biopsies

Een innovatieve, niet-invasieve strategie voor mutatieanalyse bij multipel myeloom: liquid biopsies op de weg naar gepersonaliseerde geneeskunde

Liquid Biopsy-Derived DNA Sources as Tools for Comprehensive Mutation Profiling in Multiple Myeloma: A Comparative Study

PhD Fellowship Strategic Basic Research

Cite this

Liquid Biopsies as Non-Invasive Tools for Mutation Profiling in Multiple Myeloma: Application Potential, Challenges, and Opportunities

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Projects

FWOSB118: Tumor characterization and disease follow-up in multiple myeloma by (epi) genetic profiling using liquid biopsies

Research output

Een innovatieve, niet-invasieve strategie voor mutatieanalyse bij multipel myeloom: liquid biopsies op de weg naar gepersonaliseerde geneeskunde

Liquid Biopsy-Derived DNA Sources as Tools for Comprehensive Mutation Profiling in Multiple Myeloma: A Comparative Study

Prizes

PhD Fellowship Strategic Basic Research

Cite this