Liver-specific miR-122 does not affect mRNA levels of cytochrome P450 enzymes and nuclear receptors in primary rat hepatocyte cultures

Jennifer Bolleyn, Joery De Kock, Helene Mertens, Joanna Fraczek, Mathieu Vinken, Olivier Govaere, Tania Roskams, Vera Rogiers, Tamara Vanhaecke

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Abstract

Primary hepatocyte cultures suffer from the loss of xenobiotic biotransformation capacity as a function of culture time. Over the years, the different strategies developed to phenotypically stabilize these cultures have met with limited success. In the present study, we evaluate the modulation of microRNA (miRNA, miR,which are major determinants of gene expression at the post-transcriptional level, as a novel stabilization approach in this respect. miR-122, an acknowledged liver-specific miRNA species, was either overexpressed or inhibited, and the outcome on mRNA levels of cytochrome P450 enzymes and nuclear receptors was investigated. However, no persistent effects could be observed, suggesting that miRNA species other than miR-122 should be tested for their potential to serve as a target for innovative stabilization efforts in cultures of primary hepatocytes.
Original languageEnglish
Pages (from-to)165-172
Number of pages7
JournalALTEX
Volume1
Issue number1
Publication statusPublished - 1 Apr 2012

Keywords

  • microrna
  • mimic
  • inhibitor
  • primary hepatocyte
  • miR-122

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  • Epigen meeting

    Jennifer Bolleyn (Speaker)

    27 Feb 2014

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    Thierry Vandendriessche (Speaker)

    27 Feb 2014

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    Tamara Vanhaecke (Participant)

    27 Feb 2014

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