Measuring Early Germ-Layer Specification Bias in Human Pluripotent Stem Cells

Alexander Keller; Nuša Krivec; Christina Markouli; Claudia Spits

doi:10.1007/978-1-0716-1979-7_5

Measuring Early Germ-Layer Specification Bias in Human Pluripotent Stem Cells

Alexander Keller, Nuša Krivec, Christina Markouli, Claudia Spits

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Abstract

Human pluripotent stem cells have a wide variety of potential applications, ranging from clinical translation to in vitro disease modeling. However, there is significant variation in the potential of individual cell lines to differentiate towards each of the three germ layers as a result of (epi)genetic background, culture conditions, and other factors. We describe here in detail a methodology to evaluate this bias using short directed differentiation towards neuroectoderm, mesendoderm, and definitive endoderm in combination with quantification by RT-qPCR and immunofluorescent stains.

Original language	English
Pages (from-to)	57-72
Number of pages	16
Journal	Methods in Molecular Biology
Volume	2429
DOIs	https://doi.org/10.1007/978-1-0716-1979-7_5
Publication status	Published - 2022

Bibliographical note

Keywords

Cell Differentiation
Endoderm
Germ Layers
Humans
Neural Plate
Pluripotent Stem Cells

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10.1007/978-1-0716-1979-7_5

Spits_PAB
https://www.springernature.com/gp/open-research/policies/book-policies
Accepted author manuscript, 4.28 MBLicence: Unspecified

Cite this

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abstract = "Human pluripotent stem cells have a wide variety of potential applications, ranging from clinical translation to in vitro disease modeling. However, there is significant variation in the potential of individual cell lines to differentiate towards each of the three germ layers as a result of (epi)genetic background, culture conditions, and other factors. We describe here in detail a methodology to evaluate this bias using short directed differentiation towards neuroectoderm, mesendoderm, and definitive endoderm in combination with quantification by RT-qPCR and immunofluorescent stains.",

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AU - Krivec, Nuša

AU - Markouli, Christina

AU - Spits, Claudia

PY - 2022

Y1 - 2022

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AB - Human pluripotent stem cells have a wide variety of potential applications, ranging from clinical translation to in vitro disease modeling. However, there is significant variation in the potential of individual cell lines to differentiate towards each of the three germ layers as a result of (epi)genetic background, culture conditions, and other factors. We describe here in detail a methodology to evaluate this bias using short directed differentiation towards neuroectoderm, mesendoderm, and definitive endoderm in combination with quantification by RT-qPCR and immunofluorescent stains.

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KW - Endoderm

KW - Germ Layers

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KW - Neural Plate

KW - Pluripotent Stem Cells

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ER -

Measuring Early Germ-Layer Specification Bias in Human Pluripotent Stem Cells

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

FWOTM1016: The impact of recurrent chromosomal abnormalities on growth advantage and differentiation capacity of human pluripotent stem cells

FWOSB35: FWO-SB-Scholarship: defining the molecular basis for liver cell differentiation deficiency in human pluripotent stem cells

IWT660: Effect of chromosomal abnormalities on the differentiation potential and malignant characteristics of human embryonic stem cells

Cite this

Measuring Early Germ-Layer Specification Bias in Human Pluripotent Stem Cells

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Projects

FWOTM1016: The impact of recurrent chromosomal abnormalities on growth advantage and differentiation capacity of human pluripotent stem cells

FWOSB35: FWO-SB-Scholarship: defining the molecular basis for liver cell differentiation deficiency in human pluripotent stem cells

IWT660: Effect of chromosomal abnormalities on the differentiation potential and malignant characteristics of human embryonic stem cells

Cite this