Mesoderm-derived stem cells: the link between the transcriptome and their differentiation potential.

Joery De Kock, Mehdi Najar, Jennifer Bolleyn, Feras Albattah, Robim Marcelino Rodrigues, Karolien Buyl, Gordana Raicevic, Olivier Govaere, Steven Branson, Kesavan Meganathan, John Antonydas Gaspar, Tania Roskams, Agapios Sachinidis, Laurence Lagneaux, Tamara Vanhaecke, Vera Rogiers

Research output: Contribution to journalArticle

44 Citations (Scopus)


Human multipotent adult stem cells (hASCs) have become an attractive source for autologous cell transplantation, tissue engineering, developmental biology and the generation of human-based alternative in vitro models. Among the three germ cell layers, the mesoderm is the origin of today's most widely used and characterized hASC populations. A variety of isolated non-hematopoietic mesoderm-derived stem cell populations exists and all of them show important differences in terms of function, efficacy and differentiation potential both in vivo and in vitro. In order to better understand whether the intrinsic properties of these cells contribute to the overall differentiation potential of hASCs, we compared the global gene expression profiles of four mesoderm-derived stem cell populations: adipose-tissue derived stromal cells (hADSC), bone marrow-derived stromal cells (hBMSC), (fore)skin-derived precursor cells (hSKP) and Wharton's jelly-derived mesenchymal stem cells (hWJ). Significant differences in gene expression profiles were detected between distinct stem cell types. hSKP predominantly expressed genes involved in neurogenesis, skin and bone development, whereas hWJ and, to some extent, hBMSC showed an increased expression of genes involved in cardiovascular and liver development. Interestingly, the observed differential gene expression of distinct hASCs could be linked to existing differentiation data in which hASCs were differentiated towards specific cell types. As such, our data suggests that the intrinsic gene expression of the undifferentiated stem cells has an important impact on their overall differentiation potential as well as their application in stem cell-based research. Yet, the factors that define these intrinsic properties remain to be determined.
Original languageEnglish
Pages (from-to)3309-3323
Number of pages15
JournalStem Cells and Development
Publication statusPublished - 2012


  • bone marrow stromal cells
  • skin-derived precursor cells
  • adipose tissue-derived stromal cells
  • wharton's jelly
  • transcriptome
  • adult stem cells
  • mesoderm-derived stem cells
  • stem cell differentiation potential


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