Methods to determine the kinetic performance limit of contemporary chromatographic techniques

Research output: Contribution to journalArticle


By combining separation efficiency data as a function of flow rate with the column permeability, the kinetic plot method allows to determine the limits of separation power (time vs. efficiency) of different chromatographic techniques and methods. The technique can be applied for all different types of chromatography (liquid, gas, or supercritical fluid), for different types of column morphologies (packed beds, monoliths, open tubular, micromachined columns), for pressure and electro-driven separations and in both isocratic and gradient elution mode. The present contribution gives an overview of the methods and calculations required to correctly determine these kinetic performance limits and their underlying limitations.
Original languageEnglish
Pages (from-to)323-339
Number of pages17
JournalJournal of Separation Science
Issue number1
Early online date4 Oct 2020
Publication statusPublished - Jan 2021


  • gas chromatography
  • kinetic performance limit
  • kinetic plot method
  • liquid chromatography
  • supercritical fluid chromatography

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