Molecular architecture of the endocytic TPLATE complex

Klaas Yperman; Jie Wang; Dominique Eeckhout; Joanna Winkler; Lam Dai Vu; Michael Vandorpe; Peter Grones; Evelien Mylle; Michael Kraus; Romain Merceron; Jonah Nolf; Eliana Mor; Pieter De Bruyn; Remy Loris; Martin Potocký; Savvas Savvides; Bert De Rybel; Geert De Jaeger; Daniel Van Damme; Roman Pleskot

doi:10.1126/sciadv.abe7999

Molecular architecture of the endocytic TPLATE complex

Klaas Yperman, Jie Wang, Dominique Eeckhout, Joanna Winkler, Lam Dai Vu, Michael Vandorpe, Peter Grones, Evelien Mylle, Michael Kraus, Romain Merceron, Jonah Nolf, Eliana Mor, Pieter De Bruyn, Remy Loris, Martin Potocký, Savvas Savvides, Bert De Rybel, Geert De Jaeger, Daniel Van Damme, Roman Pleskot

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16 Citations (Scopus)

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Abstract

Eukaryotic cells rely on endocytosis to regulate their plasma membrane proteome and lipidome. Most eukaryotic groups, except fungi and animals, have retained the evolutionary ancient TSET complex as endocytic regulator. Unlike other coatomer complexes, structural insight into TSET is lacking. Here, we reveal the molecular architecture of plant TSET (TPLATE complex/TPC) using an integrative structural approach. We identify crucial roles for specific TSET subunits in complex assembly and membrane interaction. Our data therefore generates novel evolutionary insight into the difference between the hexameric TSET in Dictyostelium and the octameric TPC in plants. Structural elucidation of this ancient adaptor complex represents the missing piece in the coatomer-puzzle and vastly advances our functional as well as evolutionary insight into the process of endocytosis.

Original language	English
Article number	eabe7999
Number of pages	16
Journal	Science Advances
Volume	7
Issue number	9
DOIs	https://doi.org/10.1126/sciadv.abe7999
Publication status	Published - 24 Feb 2021

Keywords

Structural Biology
Plant molecular biology
CD spectroscopy
Endocytosis

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10.1126/sciadv.abe7999Licence: CC BY-NC

Molecular architecture of the endocytic TPLATE complexFinal published version, 8.15 MBLicence: CC BY-NC

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16 Citations
1 PhD Thesis

Toxin-antitoxin modules: functional characterization of transcription regulation and potential use against pathogenic bacteria
De Bruyn, P., 2021, Brussels.
Research output: Thesis › PhD Thesis

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Yperman, K., Wang, J., Eeckhout, D., Winkler, J., Vu, L. D., Vandorpe, M., Grones, P., Mylle, E., Kraus, M., Merceron, R., Nolf, J., Mor, E., De Bruyn, P., Loris, R., Potocký, M., Savvides, S., De Rybel, B., De Jaeger, G., Van Damme, D., & Pleskot, R. (2021). Molecular architecture of the endocytic TPLATE complex. Science Advances, 7(9), [eabe7999]. https://doi.org/10.1126/sciadv.abe7999

Yperman, Klaas ; Wang, Jie ; Eeckhout, Dominique ; Winkler, Joanna ; Vu, Lam Dai ; Vandorpe, Michael ; Grones, Peter ; Mylle, Evelien ; Kraus, Michael ; Merceron, Romain ; Nolf, Jonah ; Mor, Eliana ; De Bruyn, Pieter ; Loris, Remy ; Potocký, Martin ; Savvides, Savvas ; De Rybel, Bert ; De Jaeger, Geert ; Van Damme, Daniel ; Pleskot, Roman. / Molecular architecture of the endocytic TPLATE complex. In: Science Advances. 2021 ; Vol. 7, No. 9.

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title = "Molecular architecture of the endocytic TPLATE complex",

abstract = "Eukaryotic cells rely on endocytosis to regulate their plasma membrane proteome and lipidome. Most eukaryotic groups, except fungi and animals, have retained the evolutionary ancient TSET complex as endocytic regulator. Unlike other coatomer complexes, structural insight into TSET is lacking. Here, we reveal the molecular architecture of plant TSET (TPLATE complex/TPC) using an integrative structural approach. We identify crucial roles for specific TSET subunits in complex assembly and membrane interaction. Our data therefore generates novel evolutionary insight into the difference between the hexameric TSET in Dictyostelium and the octameric TPC in plants. Structural elucidation of this ancient adaptor complex represents the missing piece in the coatomer-puzzle and vastly advances our functional as well as evolutionary insight into the process of endocytosis.",

keywords = "Structural Biology, Plant molecular biology, CD spectroscopy, Endocytosis",

author = "Klaas Yperman and Jie Wang and Dominique Eeckhout and Joanna Winkler and Vu, {Lam Dai} and Michael Vandorpe and Peter Grones and Evelien Mylle and Michael Kraus and Romain Merceron and Jonah Nolf and Eliana Mor and {De Bruyn}, Pieter and Remy Loris and Martin Potock{\'y} and Savvas Savvides and {De Rybel}, Bert and {De Jaeger}, Geert and {Van Damme}, Daniel and Roman Pleskot",

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doi = "10.1126/sciadv.abe7999",

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Yperman, K, Wang, J, Eeckhout, D, Winkler, J, Vu, LD, Vandorpe, M, Grones, P, Mylle, E, Kraus, M, Merceron, R, Nolf, J, Mor, E, De Bruyn, P , Loris, R, Potocký, M, Savvides, S, De Rybel, B, De Jaeger, G, Van Damme, D & Pleskot, R 2021, 'Molecular architecture of the endocytic TPLATE complex', Science Advances, vol. 7, no. 9, eabe7999. https://doi.org/10.1126/sciadv.abe7999

Molecular architecture of the endocytic TPLATE complex. / Yperman, Klaas; Wang, Jie; Eeckhout, Dominique; Winkler, Joanna; Vu, Lam Dai; Vandorpe, Michael; Grones, Peter; Mylle, Evelien; Kraus, Michael; Merceron, Romain; Nolf, Jonah; Mor, Eliana; De Bruyn, Pieter ; Loris, Remy; Potocký, Martin; Savvides, Savvas; De Rybel, Bert; De Jaeger, Geert; Van Damme, Daniel; Pleskot, Roman.

In: Science Advances, Vol. 7, No. 9, eabe7999, 24.02.2021.

Research output: Contribution to journal › Article › peer-review

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T1 - Molecular architecture of the endocytic TPLATE complex

AU - Yperman, Klaas

AU - Wang, Jie

AU - Eeckhout, Dominique

AU - Winkler, Joanna

AU - Vu, Lam Dai

AU - Vandorpe, Michael

AU - Grones, Peter

AU - Mylle, Evelien

AU - Kraus, Michael

AU - Merceron, Romain

AU - Nolf, Jonah

AU - Mor, Eliana

AU - De Bruyn, Pieter

AU - Loris, Remy

AU - Potocký, Martin

AU - Savvides, Savvas

AU - De Rybel, Bert

AU - De Jaeger, Geert

AU - Van Damme, Daniel

AU - Pleskot, Roman

PY - 2021/2/24

Y1 - 2021/2/24

N2 - Eukaryotic cells rely on endocytosis to regulate their plasma membrane proteome and lipidome. Most eukaryotic groups, except fungi and animals, have retained the evolutionary ancient TSET complex as endocytic regulator. Unlike other coatomer complexes, structural insight into TSET is lacking. Here, we reveal the molecular architecture of plant TSET (TPLATE complex/TPC) using an integrative structural approach. We identify crucial roles for specific TSET subunits in complex assembly and membrane interaction. Our data therefore generates novel evolutionary insight into the difference between the hexameric TSET in Dictyostelium and the octameric TPC in plants. Structural elucidation of this ancient adaptor complex represents the missing piece in the coatomer-puzzle and vastly advances our functional as well as evolutionary insight into the process of endocytosis.

AB - Eukaryotic cells rely on endocytosis to regulate their plasma membrane proteome and lipidome. Most eukaryotic groups, except fungi and animals, have retained the evolutionary ancient TSET complex as endocytic regulator. Unlike other coatomer complexes, structural insight into TSET is lacking. Here, we reveal the molecular architecture of plant TSET (TPLATE complex/TPC) using an integrative structural approach. We identify crucial roles for specific TSET subunits in complex assembly and membrane interaction. Our data therefore generates novel evolutionary insight into the difference between the hexameric TSET in Dictyostelium and the octameric TPC in plants. Structural elucidation of this ancient adaptor complex represents the missing piece in the coatomer-puzzle and vastly advances our functional as well as evolutionary insight into the process of endocytosis.

KW - Structural Biology

KW - Plant molecular biology

KW - CD spectroscopy

KW - Endocytosis

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U2 - 10.1126/sciadv.abe7999

DO - 10.1126/sciadv.abe7999

M3 - Article

C2 - 33637534

VL - 7

JO - Science Advances

JF - Science Advances

SN - 2375-2548

IS - 9

M1 - eabe7999

ER -

Molecular architecture of the endocytic TPLATE complex

Abstract

Keywords

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Research output

Toxin-antitoxin modules: functional characterization of transcription regulation and potential use against pathogenic bacteria

Cite this