Abstract
Most Ewing family tumors are identified by the characteristic translocation t(11;22)(q24;q12), resulting in a fusion protein EWS/FLI1 that acts as an aberrant transcription factor. In a minority of cases, the EWS gene is fused to another member of the ETS gene (ERG, ETV1, E1AF, and FEV). Though the oncogenic transforming capability of the EWS/FLI1 protein is highly suggestive, the exact pathway behind remains to be elucidated. The availability of cell lines may help in the understanding of underlying cellular processes. In this study, we have established two new Ewing sarcoma cell lines and characterized them with molecular cytogenetic tools. This technology was also applied on four other previously published Ewing sarcoma cell lines. Our findings in relation to previous data on similar tumors are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 173-179 |
| Number of pages | 7 |
| Journal | Cancer Genetics and Cytogenetics |
| Volume | 166 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 15 Apr 2006 |
| Externally published | Yes |
Bibliographical note
Funding Information:This project was supported by a grant from the Dutch Cancer Foundation (KWF2001-2526). We are grateful to Dr. S.A. Burchill (St. James' University Hospital, Leeds, UK) for kindly donating the TC-32, TTC-466 cell lines, Martine Peter (Laboratoire de Transfert, Institut Curie, Paris, France) for donating cell lines EW3 and EW7, and E. Kerkhof (Leiden University Medical Center, Leiden, the Netherlands) for performing the RT-PCR experiments.