Monitoring of starter culture-initiated liquid wheat and teff sourdough fermentations by selected ion flow tube-mass spectrometry

Simon Van Kerrebroeck, Henning Harth, Andrea Comasio, Luc De Vuyst

Research output: Contribution to journalArticle

10 Citations (Scopus)


BACKGROUND: Selected ion flow tube–mass spectrometry (SIFT-MS) is a direct-injection mass spectrometric technique that has been introduced recently into the field of food and flavor analysis. It also shows potential for use in themonitoring of food fermentations. Therefore, this study aimed at the online monitoring of different volatile compounds produced during starter
culture-initiated liquid sourdough fermentations by SIFT-MS, for which a new workflow was developed.
RESULTS: The online monitoring of the volatile sample compounds acetoin and ethyl acetate, diacetyl, and ethanol was made possible during the production of sourdoughs obtained through fermentation with several interesting strains belonging to the species Lactobacillus crustorum, Lactobacillus fermentum, Lactobacillus hilgardii, Lactobacillus nagelii, Lactobacillus sakei, and
Gluconobacter oxydans. Acetoin and ethyl acetate could not be distinguished based solely on SIFT-MS data. Diacetyl production wasmonitored in the case of Lb. crustorum LMG 23699 as a starter culture strain, thereby making the distinction between those volatiles produced in sourdough without extra ingredients added or after the addition of citrate ormalate.
CONCLUSION: Starter culture-initiated liquid sourdough fermentations were monitored successfully. The volatile compound production of the different starter culture strains tested reflected differences in their metabolism and/or competitiveness in a sourdough matrix.
Original languageEnglish
Pages (from-to)3501-3512
Number of pages12
JournalJournal of the Science of Food and Agriculture
Issue number9
Publication statusPublished - Jul 2018


  • diacetyl
  • ethanol
  • online monitoring
  • sourdough fermentation
  • volatile compounds


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