Abstract
BACKGROUND: In order to explore the plasticity and transdifferentiation potential of murine spermatogonial stem cells (SSCs) into hematopoietic cells.
METHODS: GFP+CD49f+H-2Kb- SSCs of male donor mice were isolated and injected into the bone marrow of Busulfan-treated GFP- female mice. Twelve weeks post transplantation, the recipients were discarded and their bone marrow, peripheral blood and spleen cells were collected and evaluated by phenotypical methods, i.e. fluorescence activated cell sorting (FACS), immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH), and functional assays, i.e. colony forming units assay (CFU) and intra-bone marrow transplantation (IBMT).
RESULTS: GFP and Y-chromosome positive cells were observed in the bone marrow, peripheral blood and spleen of transplanted female mice. These cells presented phenotypical and functional characteristics of hematopoietic cells in-vitro and in-vivo.
CONCLUSION: Our results indicate that SSCs have the potential to transdifferentiate into hematopoietic cells in-vivo.
METHODS: GFP+CD49f+H-2Kb- SSCs of male donor mice were isolated and injected into the bone marrow of Busulfan-treated GFP- female mice. Twelve weeks post transplantation, the recipients were discarded and their bone marrow, peripheral blood and spleen cells were collected and evaluated by phenotypical methods, i.e. fluorescence activated cell sorting (FACS), immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH), and functional assays, i.e. colony forming units assay (CFU) and intra-bone marrow transplantation (IBMT).
RESULTS: GFP and Y-chromosome positive cells were observed in the bone marrow, peripheral blood and spleen of transplanted female mice. These cells presented phenotypical and functional characteristics of hematopoietic cells in-vitro and in-vivo.
CONCLUSION: Our results indicate that SSCs have the potential to transdifferentiate into hematopoietic cells in-vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 3101-3109 |
| Number of pages | 9 |
| Journal | Human Reproduction |
| Volume | 25 |
| Issue number | 2010 |
| Publication status | Published - 11 Oct 2010 |
Keywords
- transdifferentiation
- spermatogonial stem cell
- hematopoietic stem cell