Multicenter Study of Cronobacter sakazakii Infections in Humans, Europe, 2017

EUCRONI Study Group; Sarah Lepuschitz; Werner Ruppitsch; Shiva Pekard-Amenitsch; Stephen J Forsythe; Martin Cormican; Robert L Mach; Denis Piérard; Franz Allerberger

doi:10.3201/eid2503.181652

Multicenter Study of Cronobacter sakazakii Infections in Humans, Europe, 2017

EUCRONI Study Group, Sarah Lepuschitz, Werner Ruppitsch, Shiva Pekard-Amenitsch, Stephen J Forsythe, Martin Cormican, Robert L Mach, Denis Piérard, Franz Allerberger

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Abstract

Cronobacter sakazakii has been documented as a cause of life-threating infections, predominantly in neonates. We conducted a multicenter study to assess the occurrence of C. sakazakii across Europe and the extent of clonality for outbreak detection. National coordinators representing 24 countries in Europe were requested to submit all human C. sakazakii isolates collected during 2017 to a study center in Austria. Testing at the center included species identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, subtyping by whole-genome sequencing (WGS), and determination of antimicrobial resistance. Eleven countries sent 77 isolates, including 36 isolates from 2017 and 41 historical isolates. Fifty-nine isolates were confirmed as C. sakazakii by WGS, highlighting the challenge of correctly identifying Cronobacter spp. WGS-based typing revealed high strain diversity, indicating absence of multinational outbreaks in 2017, but identified 4 previously unpublished historical outbreaks. WGS is the recommended method for accurate identification, typing, and detection of this pathogen.

Original language	English
Pages (from-to)	515-522
Number of pages	8
Journal	Emerging Infectious Diseases
Volume	25
Issue number	3
DOIs	https://doi.org/10.3201/eid2503.181652
Publication status	Published - Mar 2019

Keywords

Anti-Bacterial Agents/pharmacology
Cronobacter sakazakii/classification
Drug Resistance, Bacterial
Enterobacteriaceae Infections/epidemiology
Europe/epidemiology
Genome, Bacterial
Genomics/methods
History, 21st Century
Humans
Infant, Newborn
Male
Microbial Sensitivity Tests
Molecular Typing
Multilocus Sequence Typing
Phylogeny
Public Health Surveillance
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Whole Genome Sequencing

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title = "Multicenter Study of Cronobacter sakazakii Infections in Humans, Europe, 2017",

abstract = "Cronobacter sakazakii has been documented as a cause of life-threating infections, predominantly in neonates. We conducted a multicenter study to assess the occurrence of C. sakazakii across Europe and the extent of clonality for outbreak detection. National coordinators representing 24 countries in Europe were requested to submit all human C. sakazakii isolates collected during 2017 to a study center in Austria. Testing at the center included species identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, subtyping by whole-genome sequencing (WGS), and determination of antimicrobial resistance. Eleven countries sent 77 isolates, including 36 isolates from 2017 and 41 historical isolates. Fifty-nine isolates were confirmed as C. sakazakii by WGS, highlighting the challenge of correctly identifying Cronobacter spp. WGS-based typing revealed high strain diversity, indicating absence of multinational outbreaks in 2017, but identified 4 previously unpublished historical outbreaks. WGS is the recommended method for accurate identification, typing, and detection of this pathogen.",

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author = "{EUCRONI Study Group} and Sarah Lepuschitz and Werner Ruppitsch and Shiva Pekard-Amenitsch and Forsythe, {Stephen J} and Martin Cormican and Mach, {Robert L} and Denis Pi{\'e}rard and Franz Allerberger",

year = "2019",

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doi = "10.3201/eid2503.181652",

language = "English",

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pages = "515--522",

journal = "Emerging Infectious Diseases",

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publisher = "Centers for Disease Control and Prevention (CDC)",

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AU - Forsythe, Stephen J

AU - Cormican, Martin

AU - Mach, Robert L

AU - Piérard, Denis

AU - Allerberger, Franz

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AB - Cronobacter sakazakii has been documented as a cause of life-threating infections, predominantly in neonates. We conducted a multicenter study to assess the occurrence of C. sakazakii across Europe and the extent of clonality for outbreak detection. National coordinators representing 24 countries in Europe were requested to submit all human C. sakazakii isolates collected during 2017 to a study center in Austria. Testing at the center included species identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, subtyping by whole-genome sequencing (WGS), and determination of antimicrobial resistance. Eleven countries sent 77 isolates, including 36 isolates from 2017 and 41 historical isolates. Fifty-nine isolates were confirmed as C. sakazakii by WGS, highlighting the challenge of correctly identifying Cronobacter spp. WGS-based typing revealed high strain diversity, indicating absence of multinational outbreaks in 2017, but identified 4 previously unpublished historical outbreaks. WGS is the recommended method for accurate identification, typing, and detection of this pathogen.

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KW - History, 21st Century

KW - Humans

KW - Infant, Newborn

KW - Male

KW - Microbial Sensitivity Tests

KW - Molecular Typing

KW - Multilocus Sequence Typing

KW - Phylogeny

KW - Public Health Surveillance

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Whole Genome Sequencing

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JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

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ER -

Multicenter Study of Cronobacter sakazakii Infections in Humans, Europe, 2017

Abstract

Keywords

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