Neuroprotective effects of the insulin-like growth factor IGF-I and 17β-estradiol after transient focal cerebral ischemia in the endothelin-1 rat model.

Wendy Stoop; Deborah De Geyter; Sophie Sarre; Ron Kooijman

Abstract

NEUROPROTECTIVE EFFECTS OF THE INSULIN-LIKE GROWTH FACTOR IGF-I AND 17β-ESTRADIOL AFTER TRANSIENT FOCAL CEREBRAL ISCHEMIA IN THE ENDOTHELIN-1 RAT MODEL.
Stoop Wendy1, De Geyter Deborah1, Sarre Sophie2, Kooijman Ron1.
1 Department of Pharmacology, 2 Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neuroscience, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.

Introduction: Reperfusion by thrombolysis is the only approved therapy for acute ischemic stroke but, only a minority of patients benefit from this treatment. IGF-I is an anti-apoptotic pleiotropic factor exerting effects on different levels of the ischemic cascade. The neuroprotective effects of the IGF-I and oestrogen receptors are interdependent. This phenomenon may allow to increase the efficacy of IGF-I by co-treatment with estradiol.

Aim: Possible synergistic effects of IGF-I and 17β-estradiol (E2) will be studied in the endothelin (Et)-1 rat model for stroke. Effects on infarct size, motor/sensory deficits and microglial activation were investigated.

Material and methods: Et-1 was administered in the vicinity of the middle cerebral artery of male albino Wistar Kyoto (WKY) rats. Rats were treated with E2 (1mg/kg, s.c.) and/or IGF-I (3mg/kg, s.c.) 30 min after induction of stroke. Motor/sensory functions were determined 24 h after the insult. Infarct size and microglial activation were assessed using cresylviolet staining and immunohistochemistry.
Results: Rats treated with E2 or IGF-I alone showed smaller infarct sizes when compared to vehicle treated animals. Treatment with a combination of E2 and IGF-I 30 min after the induction of stroke resulted in a more pronounced reduction in infarct size and an increase in round shaped activated microglia. The NDS was significantly reduced in vehicle-treated animals but not in rats treated with E2 and/or IGF-I.
Conclusion: A combination of both E2 and IGF-I reduces infarct size after a transient focal cerebral ischemia and increased the number of round shaped microglial cells.

Original language	English
Title of host publication	-10th Meeting of the Belgian Society for Neuroscience
Publication status	Published - 31 May 2013
Event	10th Meeting of the Belgian Society for Neuroscience - Brussels, Belgium Duration: 31 May 2013 → 31 May 2013

Conference

Conference	10th Meeting of the Belgian Society for Neuroscience
Country/Territory	Belgium
City	Brussels
Period	31/05/13 → 31/05/13

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@inbook{c65c3de91f384ac38083470654162318,

title = "Neuroprotective effects of the insulin-like growth factor IGF-I and 17β-estradiol after transient focal cerebral ischemia in the endothelin-1 rat model.",

abstract = "NEUROPROTECTIVE EFFECTS OF THE INSULIN-LIKE GROWTH FACTOR IGF-I AND 17β-ESTRADIOL AFTER TRANSIENT FOCAL CEREBRAL ISCHEMIA IN THE ENDOTHELIN-1 RAT MODEL.Stoop Wendy1, De Geyter Deborah1, Sarre Sophie2, Kooijman Ron1.1 Department of Pharmacology, 2 Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neuroscience, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.Introduction: Reperfusion by thrombolysis is the only approved therapy for acute ischemic stroke but, only a minority of patients benefit from this treatment. IGF-I is an anti-apoptotic pleiotropic factor exerting effects on different levels of the ischemic cascade. The neuroprotective effects of the IGF-I and oestrogen receptors are interdependent. This phenomenon may allow to increase the efficacy of IGF-I by co-treatment with estradiol.Aim: Possible synergistic effects of IGF-I and 17β-estradiol (E2) will be studied in the endothelin (Et)-1 rat model for stroke. Effects on infarct size, motor/sensory deficits and microglial activation were investigated.Material and methods: Et-1 was administered in the vicinity of the middle cerebral artery of male albino Wistar Kyoto (WKY) rats. Rats were treated with E2 (1mg/kg, s.c.) and/or IGF-I (3mg/kg, s.c.) 30 min after induction of stroke. Motor/sensory functions were determined 24 h after the insult. Infarct size and microglial activation were assessed using cresylviolet staining and immunohistochemistry.Results: Rats treated with E2 or IGF-I alone showed smaller infarct sizes when compared to vehicle treated animals. Treatment with a combination of E2 and IGF-I 30 min after the induction of stroke resulted in a more pronounced reduction in infarct size and an increase in round shaped activated microglia. The NDS was significantly reduced in vehicle-treated animals but not in rats treated with E2 and/or IGF-I. Conclusion: A combination of both E2 and IGF-I reduces infarct size after a transient focal cerebral ischemia and increased the number of round shaped microglial cells. ",

author = "Wendy Stoop and {De Geyter}, Deborah and Sophie Sarre and Ron Kooijman",

year = "2013",

month = may,

day = "31",

language = "English",

booktitle = "-10th Meeting of the Belgian Society for Neuroscience",

note = "10th Meeting of the Belgian Society for Neuroscience ; Conference date: 31-05-2013 Through 31-05-2013",

}

Neuroprotective effects of the insulin-like growth factor IGF-I and 17β-estradiol after transient focal cerebral ischemia in the endothelin-1 rat model. / Stoop, Wendy; De Geyter, Deborah; Sarre, Sophie; Kooijman, Ron.

-10th Meeting of the Belgian Society for Neuroscience. 2013.

Research output: Chapter in Book/Report/Conference proceeding › Meeting abstract (Book) › Research

TY - CHAP

T1 - Neuroprotective effects of the insulin-like growth factor IGF-I and 17β-estradiol after transient focal cerebral ischemia in the endothelin-1 rat model.

AU - Stoop, Wendy

AU - De Geyter, Deborah

AU - Sarre, Sophie

AU - Kooijman, Ron

PY - 2013/5/31

Y1 - 2013/5/31

N2 - NEUROPROTECTIVE EFFECTS OF THE INSULIN-LIKE GROWTH FACTOR IGF-I AND 17β-ESTRADIOL AFTER TRANSIENT FOCAL CEREBRAL ISCHEMIA IN THE ENDOTHELIN-1 RAT MODEL.Stoop Wendy1, De Geyter Deborah1, Sarre Sophie2, Kooijman Ron1.1 Department of Pharmacology, 2 Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neuroscience, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.Introduction: Reperfusion by thrombolysis is the only approved therapy for acute ischemic stroke but, only a minority of patients benefit from this treatment. IGF-I is an anti-apoptotic pleiotropic factor exerting effects on different levels of the ischemic cascade. The neuroprotective effects of the IGF-I and oestrogen receptors are interdependent. This phenomenon may allow to increase the efficacy of IGF-I by co-treatment with estradiol.Aim: Possible synergistic effects of IGF-I and 17β-estradiol (E2) will be studied in the endothelin (Et)-1 rat model for stroke. Effects on infarct size, motor/sensory deficits and microglial activation were investigated.Material and methods: Et-1 was administered in the vicinity of the middle cerebral artery of male albino Wistar Kyoto (WKY) rats. Rats were treated with E2 (1mg/kg, s.c.) and/or IGF-I (3mg/kg, s.c.) 30 min after induction of stroke. Motor/sensory functions were determined 24 h after the insult. Infarct size and microglial activation were assessed using cresylviolet staining and immunohistochemistry.Results: Rats treated with E2 or IGF-I alone showed smaller infarct sizes when compared to vehicle treated animals. Treatment with a combination of E2 and IGF-I 30 min after the induction of stroke resulted in a more pronounced reduction in infarct size and an increase in round shaped activated microglia. The NDS was significantly reduced in vehicle-treated animals but not in rats treated with E2 and/or IGF-I. Conclusion: A combination of both E2 and IGF-I reduces infarct size after a transient focal cerebral ischemia and increased the number of round shaped microglial cells.

AB - NEUROPROTECTIVE EFFECTS OF THE INSULIN-LIKE GROWTH FACTOR IGF-I AND 17β-ESTRADIOL AFTER TRANSIENT FOCAL CEREBRAL ISCHEMIA IN THE ENDOTHELIN-1 RAT MODEL.Stoop Wendy1, De Geyter Deborah1, Sarre Sophie2, Kooijman Ron1.1 Department of Pharmacology, 2 Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neuroscience, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.Introduction: Reperfusion by thrombolysis is the only approved therapy for acute ischemic stroke but, only a minority of patients benefit from this treatment. IGF-I is an anti-apoptotic pleiotropic factor exerting effects on different levels of the ischemic cascade. The neuroprotective effects of the IGF-I and oestrogen receptors are interdependent. This phenomenon may allow to increase the efficacy of IGF-I by co-treatment with estradiol.Aim: Possible synergistic effects of IGF-I and 17β-estradiol (E2) will be studied in the endothelin (Et)-1 rat model for stroke. Effects on infarct size, motor/sensory deficits and microglial activation were investigated.Material and methods: Et-1 was administered in the vicinity of the middle cerebral artery of male albino Wistar Kyoto (WKY) rats. Rats were treated with E2 (1mg/kg, s.c.) and/or IGF-I (3mg/kg, s.c.) 30 min after induction of stroke. Motor/sensory functions were determined 24 h after the insult. Infarct size and microglial activation were assessed using cresylviolet staining and immunohistochemistry.Results: Rats treated with E2 or IGF-I alone showed smaller infarct sizes when compared to vehicle treated animals. Treatment with a combination of E2 and IGF-I 30 min after the induction of stroke resulted in a more pronounced reduction in infarct size and an increase in round shaped activated microglia. The NDS was significantly reduced in vehicle-treated animals but not in rats treated with E2 and/or IGF-I. Conclusion: A combination of both E2 and IGF-I reduces infarct size after a transient focal cerebral ischemia and increased the number of round shaped microglial cells.

M3 - Meeting abstract (Book)

BT - -10th Meeting of the Belgian Society for Neuroscience

T2 - 10th Meeting of the Belgian Society for Neuroscience

Y2 - 31 May 2013 through 31 May 2013

ER -

Neuroprotective effects of the insulin-like growth factor IGF-I and 17β-estradiol after transient focal cerebral ischemia in the endothelin-1 rat model.

Abstract

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