Next-generation sequencing to enhance the taxonomic resolution of the microbiological analysis of meat and meat-derived products

Emiel Niels Van Reckem, Luc De Vuyst, Stefan Weckx, Frederic Leroy

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)
6 Downloads (Pure)

Abstract

Meat makes up a complex environment, home to an extensive bacterial diversity that is heavily influenced by environmental conditions during processing and storage. The current review focuses on the use of next-generation sequencing methods to unravel the complex microbial communities that are present in meat environments in greater detail. Recent applications of these methods in amplicon-sequencing approaches targeting other phylogenetic marker genes than the 16S rRNA gene allowed for a better assessment of species richness in meat and the detection of many minor communities that might have previously gone underreported. With the increasing base quality of the single-molecule sequencing methods, an even improved resolution is to be expected, as high-throughput sequencing of full-length phylogenetic marker genes is on the RISE.
Original languageEnglish
Pages (from-to)58-65
Number of pages8
JournalCurrent Opinion in Food Science
Volume37
DOIs
Publication statusPublished - Feb 2021

Bibliographical note

Funding Information:
This work was supported by the Research Council of the Vrije Universiteit Brussel (SRP7, IOF342, and IRP11 projects), the Hercules Foundation (project UABR 09/004) and the Research Foundation-Flanders (G021518N). E.V.R. is the recipient of a SB Fellowship of the FWO (1S06717N).

Funding Information:
This work was supported by the Research Council of the Vrije Universiteit Brussel (SRP7, IOF342, and IRP11 projects), the Hercules Foundation (project UABR 09/004) and the Research Foundation-Flanders ( G021518N ). E.V.R. is the recipient of a SB Fellowship of the FWO ( 1S06717N ).

Publisher Copyright:
© 2020 Elsevier Ltd

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

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