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(EDCs) or endocrine active chemicals (EACs) can occur at relatively low
concentrations in the aquatic environment. Monitoring these compounds as
encouraged by the European Water Framework Directive (WFD, 2013/39/EU)
demands efficient, simple and sensitive techniques to detect and quantify
these emerging pollutants of concern (EPOCs).
Here, a novel sampling technique of diffusive gradients in thin films (DGT)
combined with the estrogen responsive element - chemically activated
luciferase gene expression (ERE-CALUX) bioassay was developed for in situ
pre-concentration and estrogenic effect measurement of steroids in the
aquatic environment at environmentally relevant levels. In this study, the
performance of this novel method was assessed, with 17β-estradiol (E2) as
a model estrogen in a single compound solution, VM7Luc4E2 cells (formerly
BG1Luc4E2) as ERE-CALUX bioassay cells and XAD 18 resin gel as a binding
Laboratory tests showed that DGT components and the experimental matrix
do not influence the estrogenic activity of ERE-CALUX cells. XAD 18 showed
sufficiently high capacity for the binding of E2 and that the measured
effective diffusion coefficient of E2 in an agarose diffusive gel was 4.7 ×
10-6 cm2/s at 25°C. The detection limit of this novel method was significantly
lower than the one obtained from active sampling combined with GC/MS
and LC/MS analysis (0.1-7.0 ng/L) described in literature and reached 0.026
± 0.003 ng/L of E2 for a one-day deployment. The method was independent
of pH (5-8), ionic strength (0.001-0.1M) and dissolved organic matter
concentrations (0-30 mg/L). The combination of diffusive gradients in thin
films with ERE-CALUX is an effective tool for pre-concentrating steroid
estrogens and possibly also in the monitoring of estrogenic activity in
|Publication status||Published - 21 Feb 2018|
|Event||ChemCYS 2018 - Floréal, Koning Albert-I-laan 59, Blankenberge, Blankenberge, Belgium|
Duration: 21 Feb 2018 → 23 Feb 2018
|Period||21/02/18 → 23/02/18|
FingerprintDive into the research topics of 'Novel passive sampling for steroid hormones in aquatic environments using DGT and the ERE-CALUX bioassay - Poster @ ChemCYS 2018'. Together they form a unique fingerprint.
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- 1 Article
Estrogenic Activity Measurements in Water Using Diffusive Gradients in Thin-Film Coupled with an Estrogen BioassayGuo, W., Van Langenhove, K., Denison, M. S., Baeyens, W., Elskens, M. & Gao, Y., 20 Nov 2017, In: Analytical Chemistry. 89, 24, p. 13357-13364 8 p.
Research output: Contribution to journal › Article › peer-review30 Citations (Scopus)
- 2 Participation in conference
Van Langenhove, Kersten (Recipient), 23 Feb 2018
Prize: Prize (including medals and awards)File