Oligoclonal bands in cerebrospinal fluid detected by PhastSystem isoelectric focusing.

Ingrid Wybo, Marianne Van Blerk, Irina Malfait, Patrick Goubert, Frans Gorus

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Pharmacia's "PhastSystem" for semi-automated isoelectric focusing (IEF) in thin precast polyacrylamide gels (PAGE) was found to be as sensitive as high-resolution protein electrophoresis (HRPE) in agarose gels and conventional PAGE-IEF for detection of oligoclonal banding (OB) in concentrated cerebrospinal fluid (CSF) samples. Both PhastSystem IEF and HRPE revealed OB in CSF from eight of nine multiple sclerosis patients and four of 10 patients with various types of infection of the central nervous system as opposed to only two of 70 patients with miscellaneous neuropsyhiatric disorders. The PhastSystem also frequently detected OB in silver-stained, unconcentrated CSF from patients with multiple sclerosis.
Original languageEnglish
Pages (from-to)123-125
Number of pages3
JournalClinical Chemistry
Publication statusPublished - 1990


  • multiple sclerosis
  • demyelinating disease
  • agarose electrophoresis
  • Coomassie Blue staining
  • silver staining


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