Optimisation of the therapeutic window of post-ischemic mild hypothermia in the endothelin-1 rat model for transient focal cerebral ischemia

Tine Zgavc, An-Gaelle Ceulemans, Said Hachimi-Idrissi, Ron Kooijman, Sophie Sarre, Yvette Michotte

Research output: Chapter in Book/Report/Conference proceedingMeeting abstract (Book)

Abstract

Background: Hypothermia is a proven neuroprotective therapy in ischemic insult. However, the time frame in which mild hypothermia still is beneficial remains unanswered. The aim of this study is to optimize the therapeutic window of mild hypothermia in the endothelin-1 rat model for focal cerebral ischemia. We compared the neuroprotective effects of hypothermia started 20 minutes after the ischemic insult versus hypothermia started after 60 or 120 minutes.
Materials and methods: Endothelin-1, a potent vasoconstrictor, was infused near the middle cerebral artery of male Wistar rats. Brain temperature was kept either under normothermic (37°C) or under hypothermic conditions (33°C) for 2 hours starting with a delay of 20, 60 or 120 minutes after the insult. The effects on neurological outcome (neurological deficit score), infarct size and apoptosis (caspase-3 immunohistochemistry) were evaluated, 24 hours after the insult.
Results: Hypothermia, initiated 20 or 60 minutes after the insult, improved neurological outcome compared to normothermic rats, whereas after 120 minutes, no improvement was observed. Compared with the normothermic group, a reduction in infarct size by 45% and 35% and in caspase-3 positive cells by 50% and 45% were observed, when hypothermia was initiated 20 and 60 minutes after the insult respectively. However, when hypothermia was delayed up to 120 minutes, no improvement was observed.
Conclusion: These findings indicate that in the endothelin-1 model, hypothermia remains neuroprotective even when the initiation of the treatment is delayed for 60 minutes after the insult, but not when delayed for 120 minutes.
Original languageEnglish
Title of host publicationProgram book of the "3rd International Hypothermia Symposium"
EditorsHans Friberg, Tadeusz Wieloch
Pages8-8
Number of pages1
Publication statusPublished - 2 Sep 2009
EventFinds and Results from the Swedish Cyprus Expedition: A Gender Perspective at the Medelhavsmuseet - Stockholm, Sweden
Duration: 21 Sep 200925 Sep 2009

Publication series

NameProgram book of the "3rd International Hypothermia Symposium"

Conference

ConferenceFinds and Results from the Swedish Cyprus Expedition: A Gender Perspective at the Medelhavsmuseet
CountrySweden
CityStockholm
Period21/09/0925/09/09

Bibliographical note

Hans Friberg and Tadeusz Wieloch

Keywords

  • Hypothermia
  • cerebral ischemia
  • Endothelin-1
  • Caspase-3

Fingerprint Dive into the research topics of 'Optimisation of the therapeutic window of post-ischemic mild hypothermia in the endothelin-1 rat model for transient focal cerebral ischemia'. Together they form a unique fingerprint.

Cite this